We narrowed to 11,231 results for: SHA

1. pRegev_D4

   Plasmid

   #204184

   Purpose

   Single reporter with long guanine depleted (G-poor) region from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   D4 exon in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRegev_D2

   Plasmid

   #204180

   Purpose

   Single reporter with long guanine depleted (G-poor) region from splicing library.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon D2 in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   61C>G mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRegev_S5.49C>A.59G>U

   Plasmid

   #204177

   Purpose

   pRegev_S5 with double mutations that restore secondary structure.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Exon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   49C>A and 59G>U mutations

   Promoter

   Truncated CAG

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRegev_ExonSkip_Backbone

   Plasmid

   #204187

   Purpose

   Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   First exon and intron for beta globin splicing minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated CAG

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BASIC_SEVA_69.11

   Plasmid

   #194214

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGL0_15 [CEN6/ARSH4 ORI]

   Plasmid

   #198928

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   CEN6/ARSH4 ORI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL2_175

   Plasmid

   #199107

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   Dummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL2_172

   Plasmid

   #199104

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVRC-N-Venus-V150A-CC-Di

   Plasmid

   #190707

   Purpose

   Encodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.

   Depositor

   Nigel Savery

   Article

   Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

   Insert

   N-Venus-V150A-CC-Di
   

   Use

   Tags

   Expression

   Mutation

   V150A

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVRC-N-Venus-V150A

    Plasmid

    #190708

    Purpose

    Control plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.

    Depositor

    Nigel Savery

    Article

    Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

    Insert

    N-Venus-V150A
    

    Use

    Tags

    Expression

    Mutation

    V150A

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR

    Plasmid

    #186276

    Purpose

    Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira shortened CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CSM160

    Plasmid

    #105684

    Purpose

    Plasmid containing an RFP dropout used to construct randomized PAM library

    Depositor

    Chase Beisel

    Article

    Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

    Insert

    RFP Golden Gate dropout
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_R0040 TetR Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. IBMc318

    Plasmid

    #161941

    Purpose

    Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-G-ER-LBD-S-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLsCas13a-gRNA-3

    Plasmid

    #164867

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLsCas13a-gRNA-2

    Plasmid

    #164866

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 Puro shRNA PIK3C2G KD1

    Plasmid

    #162008

    Purpose

    PIK3C2gamma shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    PIK3C2G (PIK3C2G Canis Lupus)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 Puro shRNA SNX9 KD1

    Plasmid

    #162014

    Purpose

    SNX9 shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (SNX9 Canis Lupus)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 Puro shRNA SNX9 KD2

    Plasmid

    #162015

    Purpose

    SNX9 shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    SNX9 (SNX9 Canis Lupus)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge