We narrowed to 5,331 results for: pcdna3.1

1. pJHG003

   Plasmid

   #126454

   Purpose

   Expresses Cas9-XTEN-dTdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-dTdT (DNTT Human, Streptococcus pyogenes)
   

   Expression

   Mammalian

   Mutation

   Added D343E and D345E to inactivate TdT.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTBL469

   Plasmid

   #126458

   Purpose

   Expresses Cas9-15xFlag-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   15x Flag
   TdT (DNTT Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACE2v2

   Plasmid

   #145149

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. aL-L3

   Plasmid

   #103881

   Purpose

   full-length human aL with moxsynGFP in betapropeller

   Depositor

   Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

   Insert

   full-length human aL with moxsynGFP in betapropeller (ITGAL Human)
   

   Tags

   tension sensor module

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LoxP3-Mb2-HA-mTFP1-2A (SO157)

   Plasmid

   #99615

   Purpose

   To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2-HA-mTfp1
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mVenus-proα2(I)-G610C

   Plasmid

   #119839

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mApple-proα2(I)

   Plasmid

   #119840

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

   Plasmid

   #210504

   Purpose

   expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
   

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FLAG-mTET2 (N500)

   Plasmid

   #89897

   Purpose

   Expresses mouse TET2 N500 truncation in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-term 500 truncation of mouse TET2 (contains fir…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-sACE2v1-Fc(IgA1)

    Plasmid

    #145155

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sACE2v2-Fc(IgA1)

    Plasmid

    #145157

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE4566

    Plasmid

    #88904

    Purpose

    Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACE2v3-sfGFP

    Plasmid

    #145174

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-sACE2v6-Fc(IgG1)

    Plasmid

    #145169

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 6

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TrpV1 scFv [N221/17]

    Plasmid

    #182082

    Purpose

    Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)
    

    Tags

    Sortase, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-sACE2v6-Fc(IgA1)

    Plasmid

    #145161

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CFP-GIV-CT FA

    Plasmid

    #69533

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV w…

    Promoter

    CMV

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

    Plasmid

    #166809

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-sACE2v3-Fc(IgG1)

    Plasmid

    #145166

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 3

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACE2v5-sfGFP

    Plasmid

    #145176

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge