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pDONR223_GATA3_WT

Plasmid

#81902

Purpose

Gateway Donor vector containing GATA3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GATA3 (GATA3 Human)

Use

Gateway entry vector

Mutation

V3A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_WT

Plasmid

#81734

Purpose

Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT3_p.Y705F

Plasmid

#82086

Purpose

Gateway Donor vector containing STAT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway entry vector

Mutation

Y705F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRSET-iSeroSnFR

Plasmid

#128483

Purpose

Fluorescent reporter for serotonin (bacterial expression)

Depositor

Lin Tian

Article

Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

Insert

iSeroSnFR

Tags

His, T7, and cpsfGFP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-TdTomato

Plasmid

#191203

Purpose

FlpO dependent TdTomato expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

TdTomato

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-SHLD3

Plasmid

#114123

Purpose

Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLJM5-mSHMT1

Plasmid

#83904

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Serine hydroxymethyltransferase 1 (Shmt1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_SNAI1_WT

Plasmid

#82164

Purpose

Gateway Donor vector containing SNAI1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SNAI1 (SNAI1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BTK_WT

Plasmid

#82197

Purpose

Gateway Donor vector containing BTK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BTK (BTK Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CD40_WT

Plasmid

#82198

Purpose

Gateway Donor vector containing CD40 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CD40 (CD40 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-PSMD2

Plasmid

#161917

Purpose

Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF4_WT

Plasmid

#82190

Purpose

Gateway Donor vector containing ATF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF4 (ATF4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DLL1_WT

Plasmid

#82108

Purpose

Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DLL1 (DLL1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3

Plasmid

#136364

Purpose

Expresses epitope-tagged human TET3 WT in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-dnMCAK

Plasmid

#208650

Purpose

Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

Depositor

Samuel Bakhoum

Article

Li et al Nature. 2023 Aug;620(7976):1080-1088. do

Insert

Kif2c (Kif2c Cricetulus griseus (Chinese hamster))

Tags

eGFP

Expression

Mammalian

Mutation

S92A , S106A , S108A, S112A , S186A, H530A, R534A…

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_FYN_WT

Plasmid

#82211

Purpose

Gateway Donor vector containing FYN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FYN (FYN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pACEMam2-TRPML1

Plasmid

#238303

Purpose

Expression of human TRPML1 in mammalian cells

Depositor

Pravin Mahajan

Article

Reeks et al Structure. 2025 Aug 7;33(8):1374-1385.e7

Insert

TRPML1 (MCOLN1 Human)

Tags

8xHis-3xFLAG-TEV

Expression

Mammalian

Promoter

hybrid CAG promoter

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_WT

Plasmid

#81736

Purpose

Gateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CA (PIK3CA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CD70_WT

Plasmid

#82003

Purpose

Gateway Donor vector containing CD70, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CD70 (CD70 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge