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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Publication

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pTre2tet)::Fbxl21

Plasmid

#51890

Purpose

expression of Fbxl21

Depositor

Joseph Takahashi

Publication

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fbxl21 (Fbxl21 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV tet

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pIC242

Plasmid

#44432

Depositor

Iain Cheeseman, Arshad Desai

Publication

Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

Insert

Mob1A (MOB1A Human)

Use

Retroviral

Tags

GFP, s peptide, and tev

Expression

Mammalian

Mutation

F5L, K15R and G212R

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGFH14A5

Plasmid

#39542

Depositor

Katharina Strub

Publication

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 96-100 changed to alanine

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
3xDHFR-myc

Plasmid

#20216

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Publication

Haimovich et al Cell Rep. 2026 Sep 7;45(9):117959. doi:

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSDC803_IKZF1_mutant

Plasmid

#224113

Purpose

Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting

Depositor

Eric Fischer

Publication

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

IKZF1 (IKZF1 Human)

Tags

Flag-TEV

Expression

Insect

Mutation

Residues 140-196, Q146A and G151N

Promoter

polyhedrin

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-TAOK2

Plasmid

#172992

Purpose

constitutive expression of FLAG-tagged TAOK2

Depositor

Michael Wehr

Publication

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK2 (TAOK2 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-siRNAres_P

Plasmid

#147196

Purpose

Mammalian Expression of HsUpf1-siRNAres

Depositor

Elisa Izaurralde

Insert

HsUpf1-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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All-in-one split ADAR2(E488Q)

Plasmid

#170137

Purpose

AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Publication

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6P1 p37

Plasmid

#169015

Purpose

Expresses GST-p37 (human, codon optimized for expression in bacteria)

Depositor

Hemmo Meyer

Publication

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

p37 (UBXN2B Human)

Tags

GST

Expression

Bacterial

Mutation

sequence was codon optimized for expression in E.…

Promoter

tac

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-MUTED

Plasmid

#164629

Purpose

Expresses MUTED in mammalian cells with a GFP tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MUTED (BLOC1S5 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Publication

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mVenus

Expression

Mammalian

Mutation

1-155-I152L

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28-MHL: nsp10_SARS-CoV-2

Plasmid

#167851

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp10

Depositor

Cheryl Arrowsmith

Publication

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLJM1 FLCN (F118D) FLAG GFP

Plasmid

#164979

Purpose

Lentiviral vector for FLCN expression in mammalian cells

Depositor

Roberto Zoncu

Publication

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Use

Lentiviral

Tags

1xFLAG-GFP

Expression

Mammalian

Mutation

F118D

Promoter

UBC

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138731

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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myc-AP4 Epsilon

Plasmid

#127623

Purpose

N-terminal myc-tagged AP4 epsilon subunit

Depositor

Josef Kittler

Publication

Ivankovic et al Autophagy. 2019 May 29:1-17. doi: 10.108

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AP-4 complex subunit epsilon-1 (AP4E1 Human)

Tags

myc

Expression

Mammalian

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF10wu_nostop

Plasmid

#152986

Purpose

Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF10wu

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF9Bwu_nostop

Plasmid

#149328

Purpose

Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF9Bwu

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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