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pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pMiR-E6AP-3'UTR-miR-375-mut

Plasmid

#53695

Purpose

Luciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site on E6AP 3'U…

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCEFL PRKACA HA WT

Plasmid

#249271

Purpose

Expression of PKA (PRKACA) wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA R135W

Plasmid

#249274

Purpose

Expression of PKA R135W mutant (PRKACAR135W)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

R135W

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA E171K

Plasmid

#249275

Purpose

Expression of PKA E171K mutant (PRKACAE171K)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

E171K

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA T49I

Plasmid

#249272

Purpose

Expression of PKA T49I mutant (PRKACAT49I)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

T49I

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL PRKACA HA E128K

Plasmid

#249273

Purpose

Expression of PKA T128K mutant (PRKACAE128K)

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

PRKACA (PRKACA Human)

Tags

HA

Expression

Mammalian

Mutation

E128K

Promoter

EF-1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 4)

Plasmid

#236609

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-flag-mAdrb1-mNeonGreen-WPRE

Plasmid

#223672

Purpose

AAV expression of flag-mAdrb1-mNeonGreen from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mAdrb1-mNeonGreen (Adrb1 Mouse)

Use

AAV

Tags

FLAG and mNeonGreen

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 6

Plasmid

#236623

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1831-2663

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 2

Plasmid

#236619

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-1006

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

Plasmid

#236613

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1

Plasmid

#236618

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-658

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-PDE6B-146

Plasmid

#239098

Purpose

pGL3 luciferase vector with 146bp PDE6B promoter (containing NRL response element).

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

PDE6B promoter (PDE6B Human)

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMDA5_2g)-PGKpuro2AmCherry-W

Plasmid

#208436

Purpose

Lentiviral gRNA plasmid targeting human MDA5 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Yee et al Mol Carcinog. 2025 Jun;64(6):1066-1077.

Insert

MDA5 (IFIH1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U10

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

Plasmid

#236612

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

Plasmid

#236608

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Mut-11G3F3G

Plasmid

#237626

Purpose

For overexpression of msfGFP-HMGB1-Mut-11G3F3G

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Mut-11G3F3G (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

11G3F3G

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_msfGFP-HMGB1-Shuffled 9

Plasmid

#237657

Purpose

For overexpression of msfGFP-HMGB1-Shuffled 9

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HMGB1-Shuffled 9 (HMGB1 Human)

Tags

msfGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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