We narrowed to 60,581 results for: promoter

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pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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mScarletI-Rab7

Plasmid

#112955

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-3xFLAG

Plasmid

#69837

Purpose

This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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BPK2139

Plasmid

#65776

Purpose

Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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Mito-ABKAR

Plasmid

#61509

Purpose

Expression of AMPK biosensor targeted to the mitochondria

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Mitochondrial targeting signal (DAKAP1-30)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-Kras

Plasmid

#61621

Purpose

Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4-Kras

Tags

C-terminal targeting sequence from Kras, Cerulean…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-ER-5a

Plasmid

#56455

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

ER 5a (CALR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-hPar2

Plasmid

#53228

Purpose

mammalian expression of human Par2

Depositor

Shaun Coughlin

Article

Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

Insert

Par2 (F2RL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-vCre

Plasmid

#55638

Purpose

EF1a-driven expression of VCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

vCre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Gamma-Tubulin

Plasmid

#54105

Purpose

Localization: Centrosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

R47H

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-Rab7

Plasmid

#51613

Purpose

Recruiter to Rab7-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab7

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2

Plasmid

#42512

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2

Use

Retroviral

Tags

Flag

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 T210D (Nigg LA183)

Plasmid

#41158

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

T210D

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only