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We narrowed to 4,045 results for: golden gate

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Showing: 1201 - 1250 of 4045 results

  1. pPD698 ZF172i

    Plasmid
    #138791
    Purpose
    Constitutive expression of ZF172i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF172i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138791-plasmid-map-sequence-id-320374

    Enlarge

  2. pPD699 ZF173i

    Plasmid
    #138792
    Purpose
    Constitutive expression of ZF173i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF173i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138792-plasmid-map-sequence-id-320375

    Enlarge

  3. pPD686 ZF13i

    Plasmid
    #138774
    Purpose
    Constitutive expression of ZF13i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF13i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138774-plasmid-map-sequence-id-320235

    Enlarge

  4. pPD687 ZF14i

    Plasmid
    #138775
    Purpose
    Constitutive expression of ZF14i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF14i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138775-plasmid-map-sequence-id-320244

    Enlarge

  5. pPD688 ZF21i

    Plasmid
    #138776
    Purpose
    Constitutive expression of ZF21i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF21i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138776-plasmid-map-sequence-id-320248

    Enlarge

  6. pPD689 ZF36i

    Plasmid
    #138777
    Purpose
    Constitutive expression of ZF36i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF36i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138777-plasmid-map-sequence-id-320252

    Enlarge

  7. pPD690 ZF42i

    Plasmid
    #138779
    Purpose
    Constitutive expression of ZF42i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF42i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138779-plasmid-map-sequence-id-320259

    Enlarge

  8. pPD691 ZF54i

    Plasmid
    #138781
    Purpose
    Constitutive expression of ZF54i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF54i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138781-plasmid-map-sequence-id-320262

    Enlarge

  9. pPD692 ZF55i

    Plasmid
    #138782
    Purpose
    Constitutive expression of ZF55i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF55i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138782-plasmid-map-sequence-id-320269

    Enlarge

  10. pPD693 ZF62i

    Plasmid
    #138783
    Purpose
    Constitutive expression of ZF62i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF62i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138783-plasmid-map-sequence-id-320273

    Enlarge

  11. pPD252 ZF92i

    Plasmid
    #138784
    Purpose
    Constitutive expression of ZF92i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF92i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138784-plasmid-map-sequence-id-320274

    Enlarge

  12. pPD694 ZF93i

    Plasmid
    #138785
    Purpose
    Constitutive expression of ZF93i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF93i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138785-plasmid-map-sequence-id-320275

    Enlarge

  13. pPD253 ZF97i

    Plasmid
    #138786
    Purpose
    Constitutive expression of ZF97i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF97i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138786-plasmid-map-sequence-id-320353

    Enlarge

  14. pPD695 ZF129i

    Plasmid
    #138787
    Purpose
    Constitutive expression of ZF129i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF129i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138787-plasmid-map-sequence-id-320354

    Enlarge

  15. pPD696 ZF150i

    Plasmid
    #138788
    Purpose
    Constitutive expression of ZF150i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF150i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138788-plasmid-map-sequence-id-320369

    Enlarge

  16. U9m

    Plasmid
    #121022
    Purpose
    MoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD22
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121022-plasmid-map-sequence-id-236185

    Enlarge

  17. T1m

    Plasmid
    #121028
    Purpose
    MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).
    Depositor
    Richard Murray
    Insert
    T500_noGap, short attachment (T11)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121028-plasmid-map-sequence-id-245901

    Enlarge

  18. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

    Enlarge

  19. U2m

    Plasmid
    #121015
    Purpose
    MoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD11
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121015-plasmid-map-sequence-id-236174

    Enlarge

  20. U3m

    Plasmid
    #121016
    Purpose
    MoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD13
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121016-plasmid-map-sequence-id-236176

    Enlarge

  21. U5m

    Plasmid
    #121018
    Purpose
    MoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD14
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121018-plasmid-map-sequence-id-238061

    Enlarge

  22. U6m

    Plasmid
    #121019
    Purpose
    MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD20
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121019-plasmid-map-sequence-id-238082

    Enlarge

  23. U7m

    Plasmid
    #121020
    Purpose
    MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD16
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121020-plasmid-map-sequence-id-236180

    Enlarge

  24. U8m

    Plasmid
    #121021
    Purpose
    MoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD24
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121021-plasmid-map-sequence-id-236184

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  25. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

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  26. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  27. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

    Enlarge

  28. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

    Enlarge

  29. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

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  30. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

    Enlarge

  31. V37m

    Plasmid
    #121043
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and mScarlet selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121043-plasmid-map-sequence-id-236355

    Enlarge

  32. C83m

    Plasmid
    #120994
    Purpose
    MoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pir protein required for R6K ori
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120994-plasmid-map-sequence-id-236103

    Enlarge

  33. C24m

    Plasmid
    #120964
    Purpose
    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lambda-cI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120964-plasmid-map-sequence-id-236100

    Enlarge

  34. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

    Enlarge

  35. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  36. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  37. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  38. P57m

    Plasmid
    #120949
    Purpose
    MoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pCin Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120949-plasmid-map-sequence-id-235961

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  39. pNDMS239

    Plasmid
    #248096
    Purpose
    mScarlet-I fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    mScarlet-I
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248096-plasmid-map-sequence-id-495375

    Enlarge

  40. RcP9_AraC_S170_LOV_P190_Sil_90bp

    Plasmid
    #242158
    Purpose
    Recombineering Plasmid 9. Positive control to test for successful editing using AraC S170 LOV (silent mutation of P190, 90bp retron)
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242158-plasmid-map-sequence-id-492364

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  41. pKS1604

    Plasmid
    #239528
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239528-plasmid-map-sequence-id-487894

    Enlarge

  42. pKS1606

    Plasmid
    #239529
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239529-plasmid-map-sequence-id-487896

    Enlarge

  43. pDest_PANCEd_AraC_S170_LOV_sd2_WT

    Plasmid
    #242151
    Purpose
    Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC S170 LOV
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242151-plasmid-map-sequence-id-484656

    Enlarge

  44. pVL1173

    Plasmid
    #239536
    Purpose
    Express CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239536-plasmid-map-sequence-id-479033

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  45. pN4

    Plasmid
    #233521
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1-10
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233521-plasmid-map-sequence-id-471179

    Enlarge

  46. pKS1620

    Plasmid
    #239551
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239551-plasmid-map-sequence-id-478368

    Enlarge

  47. pKS1617

    Plasmid
    #239548
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239548-plasmid-map-sequence-id-478367

    Enlarge

  48. pKS1618

    Plasmid
    #239549
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239549-plasmid-map-sequence-id-478366

    Enlarge

  49. pVL1172

    Plasmid
    #239535
    Purpose
    Express CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-mTurquoise2
    Expression
    Yeast
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239535-plasmid-map-sequence-id-476352

    Enlarge

  50. pKS1619

    Plasmid
    #239550
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239550-plasmid-map-sequence-id-475997

    Enlarge

Showing: 1201 - 1250 of 4045 results