We narrowed to 5,049 results for: golden gate
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Plasmid#139247PurposeVector that holds the linker for circuits with 9 TUsDepositorInsertLinker9
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1158 pLink 8
Plasmid#139246PurposeVector that holds the linker for circuits with 8 TUsDepositorInsertLinker8
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1157 pLink 7
Plasmid#139245PurposeVector that holds the linker for circuits with 7 TUsDepositorInsertLinker7
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1156 pLink 6
Plasmid#139244PurposeVector that holds the linker for circuits with 6 TUsDepositorInsertLinker6
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1155 pLink 5
Plasmid#139243PurposeVector that holds the linker for circuits with 5 TUsDepositorInsertLinker5
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1154 pLink 4
Plasmid#139242PurposeVector that holds the linker for circuits with 4 TUsDepositorInsertLinker4
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1153 pLink 3
Plasmid#139241PurposeVector that holds the linker for circuits with 3 TUsDepositorInsertLinker3
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1152 pLink 2
Plasmid#139240PurposeVector that holds the linker for circuits with 2 TUsDepositorInsertLinker2
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD1151 pLink 1
Plasmid#139239PurposeVector that holds the linker for circuits with 1 TUsDepositorInsertLinker1
UseSynthetic BiologyAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)
Plasmid#195049Purposeintegration cassette for ku70 deletion and expression of NAT gene under pPGI for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpPGIAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
cBEST2
Plasmid#234658PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterkasO*17tss and kasOp*Available SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
cBEST4
Plasmid#234660PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP3 and tcp830Available SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
nlsSalicS1
Plasmid#232190PurposeNovel FRET-based biosensor for salicylic acidDepositorInsertnlsSalicS1
TagsHis-tagExpressionPlantPromoterp16Available SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
cBEST3
Plasmid#234659PurposeExpresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertionDepositorInsertsspCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP3 and kasO17tssAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
nlsSalicS1-NR
Plasmid#232192PurposeNegative control for nlsSalicS1DepositorInsertnlsSalicS1-NR
TagsHis-tag and MycExpressionPlantPromoterP16Available SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KH
Plasmid#63208PurposepCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV10.KN
Plasmid#63209PurposepCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-PuroR-Z
Plasmid#218904Purposegolden gate cloningDepositorInserthPGK-PuroR
ExpressionMammalianMutation246T>A and 398T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
(K) tNOS (S)_T3 (SBE120)
Plasmid#195010Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)DepositorInsert(K) tNOS (S)
ExpressionBacterialAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(H) tNOS (I) _T2 (SBE119)
Plasmid#195006Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)DepositorInsert(H) tNOS (I)
ExpressionBacterialAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) HYG marker(R) (SBE264)
Plasmid#195013Purposehygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+HYG+tGPD
ExpressionBacterialPromoterpXYLAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(E) tNOS (F) _T1 (SBE118)
Plasmid#194997Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)DepositorInsert(E) tNOS (F)
ExpressionBacterialAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) BLE marker(R) (SBE262)
Plasmid#195012Purposebleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPDDepositorInsertpXYL+BLE+tGPD
ExpressionBacterialPromoterpXYLAvailable SinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV115.loxP
Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B-CsALSgR1.2
Plasmid#245876PurposeGolden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS geneDepositorInsertCsALSgR1.2
UseCRISPR; Golden gate entry vectorExpressionPlantPromoterAtU3Available SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-NeoR-SV40 polyA-Z
Plasmid#218909Purposegolden gate cloningDepositorInsertmPGK-Neomycine-SV40 polyA
ExpressionMammalianMutation395T>CAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
ExpressionMammalianMutation297T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-BlastR-Z
Plasmid#218907Purposegolden gate cloningDepositorInserthPGK-BlastR
ExpressionMammalianMutation246T>A and 398T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-NeoR-Z
Plasmid#218906Purposegolden gate cloningDepositorInserthPGK-NeoR
ExpressionMammalianMutation246T>A and 398T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-HygroR-Z
Plasmid#218905Purposegolden gate cloningDepositorInserthPGK-HygroR
ExpressionMammalianMutation246T>A and 398T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-BlastR-SV40 polyA-Z
Plasmid#218903Purposegolden gate cloningDepositorInsertmPGK-BlastR-SV40 polyA
ExpressionMammalianMutation2468T>CAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
(R)pGPD1(D)_P1(SBE109)
Plasmid#194993Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)DepositorInsert(R) pGPD (D)
ExpressionBacterialAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
(F)pGPD(G)_P2 (SBE110)
Plasmid#194992Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)DepositorInsert(F) pGPD (G)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pADH2 (J) P3 (SBE114)
Plasmid#187629Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)DepositorInsert(I) pADH2 (J)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pADH2 (G)_P2 (SBE113)
Plasmid#187628Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)DepositorInsert(F) pADH2 (G)
ExpressionBacterialAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)
Plasmid#195054Purposeintegration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpLDP1Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)
Plasmid#195053Purposeintegration cassette for ku70 deletion and expression of NAT gene under pFBA for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpFBAAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)
Plasmid#195052Purposeintegration cassette for ku70 deletion and expression of NAT gene under pXYL for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)
Plasmid#195051Purposeintegration cassette for ku70 deletion and expression of NAT gene under pGPD for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpGPDAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)
Plasmid#195050Purposeintegration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterizationDepositorInsertNAT
ExpressionBacterialPromoterpADH2Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pXYL (G) P1 (SBE216)
Plasmid#195017Purposepromoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)DepositorInsert(F) pXYL (G)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R) pXYL (D) P1 (SBE215)
Plasmid#195016Purposepromoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)DepositorInsert(R) pXYL (D)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R)pADH2(D)_P1(SBE214)
Plasmid#195015Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1DepositorInsert(B) pADH2 (R)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) NAT marker (R) (SBE173)
Plasmid#195014Purposenourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+NAT+tNOS
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pGPD (J)_P3 (SBE111)
Plasmid#194990Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)DepositorInsert(I) pGPD (J)
ExpressionBacterialAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only