We narrowed to 10,537 results for: Uty

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pACYC-PJ23100-dpGFP

Plasmid

#249257

Purpose

An avGFP mutant designed by DeepDE that achieved a remarkable 74.3-fold increase in activity over four rounds of evolution, far surpassing the benchmark superfolder GFP.

Depositor

Xiaofeng Yang

Article

Li et al iScience. 2025 Aug 7;28(9):113324. doi:

Insert

dpGFP

Expression

Bacterial

Promoter

PJ23100

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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PACE_AP_Neg_pBADProm

Plasmid

#242141

Purpose

pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III Negative

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_Pos_pBADProm

Plasmid

#242139

Purpose

pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PACE_AP_GIII_Titrate

Plasmid

#242140

Purpose

PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iC

Plasmid

#231418

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#232125

Purpose

A middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 / TCF3 (TCF7L1 Human)

Use

Gateway Cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iA

Plasmid

#231417

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAC13

Plasmid

#197407

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pVS1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pVS1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC23

Plasmid

#197409

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pBBR1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pBBR1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC24

Plasmid

#197410

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC25

Plasmid

#197411

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and RK2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RK2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC26

Plasmid

#197412

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and RK2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RK2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC04

Plasmid

#197400

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pBBR1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pBBR1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC06

Plasmid

#197401

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and RK2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RK2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC08

Plasmid

#197402

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and RSF1010 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RSF1010 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC10

Plasmid

#197404

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pVS1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pVS1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC11

Plasmid

#197405

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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