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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 159,238 results for: addgene

Showing: 481 - 500 of 159238 results

  1. 5U9N (PP-BRD3)

    Plasmid
    #101255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101255-plasmid-map-sequence-id-197534

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  2. 4QNY

    Plasmid
    #61958
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_331470.1:MAC04E-A10:C228347
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. PP-MAPK2 (3N9X)

    Plasmid
    #28117
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PBG-PF11_0147
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. PP-CDPK3

    Plasmid
    #28151
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDPK3
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    CAD domain (residues 425-602).
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid
    #232929
    Purpose
    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    OsTir1(F74G)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    changed Phenylalanine 74 to Glycine
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232929-plasmid-map-sequence-id-466497

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  8. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  11. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mutation
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  13. ShuttleMCP

    Plasmid
    #50959
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter
    Depositor
    James Bamburg
    Article
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    uses portion of the mouse cofilin gene as a mouse…
    Available since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    50959-plasmid-map-sequence-id-76720

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  18. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-440858

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Showing: 481 - 500 of 159238 results