We narrowed to 594 results for: prp

1. brr2-R1107A pRS313

   Plasmid

   #111415

   Purpose

   brr2-R1107A (endogenous promoter)

   Depositor

   Christine Guthrie

   Article

   Mayerle et al bioRxiv 2018. 354514

   Insert

   BRR2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R1107A aa mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINER5

   Plasmid

   #185895

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pINER2R

   Plasmid

   #185882

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINER3R

   Plasmid

   #185883

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIAeBlueHPV16LR

   Plasmid

   #185894

   Purpose

   In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIT7A6

   Plasmid

   #185896

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pINER4R

   Plasmid

   #185884

   Purpose

   In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103 HYPC WW domain #1

   Plasmid

   #104231

   Purpose

   Bacterial expression of WW domain #1 from HYPC

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   HYPC WW domain #1 (PRPF40B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. brr2-R1107M pRS313

   Plasmid

   #111417

   Purpose

   brr2-R1107M (endogenous promoter)

   Depositor

   Christine Guthrie

   Article

   Mayerle et al bioRxiv 2018. 354514

   Insert

   BRR2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R1107M aa mutation (note, not an RP allele)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103 HYPC WW domain #2

    Plasmid

    #104232

    Purpose

    Bacterial expression of WW domain #2 from HYPC

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    HYPC WW domain #2 (PRPF40B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZP06C

    Plasmid

    #232322

    Purpose

    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

    Depositor

    Chenggang Wu

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsJ

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAP84

    Plasmid

    #86554

    Purpose

    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile

    Depositor

    Wiep Klaas Smits

    Insert

    Pveg-sLucopt
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGC157

    Plasmid

    #19682

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28
    

    Use

    Entry clone

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. StarPort01

    Plasmid

    #164503

    Purpose

    To generate a stable acceptor cell line with a BxbI attP site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.

    Depositor

    Bo Huang

    Insert

    V5-SNAPtag-PDGFR_TM-T2A-Blast
    

    Use

    To generate attp acceptor cell lines for bxbi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human RPL30

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. PTPRN2

    Plasmid

    #38891

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPRN2 (PTPRN2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. StarPort02

    Plasmid

    #164504

    Purpose

    To generate a stable acceptor cell line with an improved BxbI attP-GA site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.

    Depositor

    Bo Huang

    Insert

    V5-SNAPtag-PDGFR_TM-T2A-Blast
    

    Use

    To generate attp-ga acceptor cell lines for bxbi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human RPL30

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKP2304

    Plasmid

    #167633

    Purpose

    Express TonB C18G, Q160C, F202A, W213A.

    Depositor

    Kathleen Postle

    Article

    Postle et al bioRxiv 2021.12.08.471779

    Insert

    tonB C18G, Q160C, F202A, W213A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    tonB with C18G, Q160C, F202A, W213A mutations. Pr…

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEcoFus

    Plasmid

    #187833

    Purpose

    1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression

    Depositor

    Jörg Vogel

    Article

    Ponath et al Nat Microbiol. 2021 Aug;6(8):1007-1020.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BCJJ163

    Plasmid

    #117512

    Purpose

    AtRPS5a promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pRPS5a:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5a

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGC114

    Plasmid

    #19622

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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