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pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Use

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/SECFP-hATG9A

Plasmid

#58995

Purpose

Expresses SECFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

Venus

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-basP:fLuc-TK:rLuc

Plasmid

#138369

Purpose

Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIntegrase_2

Plasmid

#60574

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_2

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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P1-EYFP-pA (Construct 5)

Plasmid

#55197

Purpose

Synthetic promoter responsive to gRNA1

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

EYFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Ade MLP

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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NMD2_lenti

Plasmid

#216221

Purpose

Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backbone

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_b-globin intron x2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-His-Pitaire-Myc

Plasmid

#26066

Depositor

Jonathon Pines

Article

Ko et al J Cell Sci. 2001 Jul . 114(Pt 14):2591-6

Insert

Pitaire (CDK12 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pMemoryArray

Plasmid

#60585

Purpose

An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

MemoryArray

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pYESir2-puro

Plasmid

#1769

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pIntegrase_7

Plasmid

#60578

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_7

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_5

Plasmid

#60577

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_5

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP2

Use

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
p119 (FUGW-UbCp-rtTA3)

Plasmid

#105171

Purpose

ubiquitin C promoter drives rtTA3 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

UbCp-rtTA3

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Use

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_8

Plasmid

#60579

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_8

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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P2-ECFP-pA (Construct 6)

Plasmid

#55198

Purpose

Synthetic promoter responsive to gRNA2

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

mCerulean

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Minimal Ade MLP

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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