We narrowed to 2,914 results for: GRI;

1. AA324

   Plasmid

   #215953

   Purpose

   Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   rev{U6_v1; DR_v0[EnAs]; sgCD46_v4[EnAs]; DR_v1[EnAs]; sgCD47_v2[EnAs]; DR_v2[EnAs]; sgCD55_v4[EnAs];DR_v3[EnAs];sgCD81_v4[EnAs]}
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHES7-NLuc-2A-tdTomato

   Plasmid

   #204348

   Purpose

   Donor vector to tag endogenous pig HES7 locus

   Depositor

   Li-Fang Chu

   Article

   Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

   Insert

   NLuc-2A-tdTomato
   

   Use

   Tags

   NanoLuc and tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-CCM2-mScarlet-I

   Plasmid

   #208886

   Purpose

   Express mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   CCM2 targeted to the mitonchondria

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIR218-2_nt5G>U

   Plasmid

   #174009

   Purpose

   miR218 nt5 point mutation in SLIT3

   Depositor

   Andrew M. Siedlecki

   Article

   Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

   Insert

   SLIT3 (SLIT3, MIR218-2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NT5 G>U point mutation in SLIT3

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

   Plasmid

   #174632

   Purpose

   Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   GFP-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

   Plasmid

   #175051

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Thr76 to Asp, phosphomimetic mutation

   Promoter

   T7 promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

   Plasmid

   #175050

   Purpose

   Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120295

   Purpose

   AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   C-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   split-intein

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC20cdc8.A18T

   Plasmid

   #99360

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC20cdc8.E31K

    Plasmid

    #99361

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 31 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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