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We narrowed to 18,127 results for: Mut

Showing: 9961 - 10000 of 18127 results

  1. pQdCas12a.sggfp(B)

    Plasmid
    #236040
    Purpose
    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236040-plasmid-map-sequence-id-469924

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  2. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #177424
    Purpose
    To express Venus fused to Noxa-4E, with a longer linker sequence between compared to Addgene #166745.
    Depositor
    David Andrews
    Insert
    Noxa, PMA-induced protein 1 (PMAIP1 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    177424-plasmid-map-sequence-id-348448

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  3. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

    Plasmid
    #227088
    Purpose
    Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H, K1355A, K1435A
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227088-plasmid-map-sequence-id-451126

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  5. pET50_G495R MDA5

    Plasmid
    #216803
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 (IFIH1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G495R
    Promoter
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216803-plasmid-map-sequence-id-428833

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  6. pFastBac1-C17orf53-Strep

    Plasmid
    #211446
    Purpose
    generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    Strep-Tag
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    211446-plasmid-map-sequence-id-420423

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  7. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available Since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  15. pcDNA3-Mcm2-mCer

    Plasmid
    #211397
    Purpose
    mammalian expression of human Mcm2 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm2 (MCM2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211397-plasmid-map-sequence-id-420430

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  16. pcDNA3-RPA32-mVen

    Plasmid
    #211388
    Purpose
    mammalian expression of human RPA32 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211388-plasmid-map-sequence-id-420425

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  17. pcDNA3-RPA32-mCer

    Plasmid
    #211387
    Purpose
    mammalian expression of human RPA32 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211387-plasmid-map-sequence-id-420424

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  18. pT7CFE1_EVA71_VP12A_C110A

    Plasmid
    #212976
    Purpose
    Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Enterovirus A71 4643 VP12A C110A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    changed cysteine 110 from 2A to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212976-plasmid-map-sequence-id-420360

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  19. pcDNA3-C17orf53_1-87-mCer

    Plasmid
    #211441
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211441-plasmid-map-sequence-id-419424

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  20. pFastBac1-C17orf53-FLAG

    Plasmid
    #211447
    Purpose
    generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-Tag
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    FLAG-Tag
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211447-plasmid-map-sequence-id-419421

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  21. pcDNA3-C17orf53_1-87-mVen

    Plasmid
    #211442
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211442-plasmid-map-sequence-id-419261

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  22. pAMW-WnkAAxV

    Plasmid
    #203980
    Purpose
    Myc6 Wnk under actin promoter for cell culture
    Depositor
    Andreas Jenny
    Insert
    Wnk (Wnk Fly)
    Use
    Tags
    6xMyc
    Expression
    Insect
    Mutation
    RFxV mutated to AAxV
    Promoter
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203980-plasmid-map-sequence-id-403221

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  23. pcDNA3.1-MS2-HA-HsMex3C_1-225_AD

    Plasmid
    #148482
    Purpose
    Mammalian Expression of HsMex3C_1-225
    Depositor
    Elisa Izaurralde
    Insert
    HsMex3C_1-225 (MEX3C Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation compared to variant 2 fro…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148482-plasmid-map-sequence-id-290930

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  24. pcDNA3.1-MS2-HA-HshnRNPH1_1-184_Z

    Plasmid
    #148121
    Purpose
    Mammalian Expression of HshnRNPH1_1-184
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPH1_1-184 (HNRNPH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148121-plasmid-map-sequence-id-290568

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  25. pcDNA3.1-MS2-HA-HshnRNPH1_185-449_Z

    Plasmid
    #148122
    Purpose
    Mammalian Expression of HshnRNPH1_185-449
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPH1_185-449 (HNRNPH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148122-plasmid-map-sequence-id-290569

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  26. pT7-EGFP-C1-HsNot1iso1-shRNAres_V

    Plasmid
    #147753
    Purpose
    Mammalian Expression of HsNot1iso1-shRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147753-plasmid-map-sequence-id-290200

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  27. pnEA-NpM-DmNot1_1-1148_Q

    Plasmid
    #147306
    Purpose
    Bacterial Expression of DmNot1_1-1148
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1_1-1148 (Not1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    4 silent mutations and two non silent N605S, K759…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147306-plasmid-map-sequence-id-289753

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  28. pAc5.1B-lambdaN-HA-DmCCR4-dsRNAres_Q

    Plasmid
    #147271
    Purpose
    Insect Expression of DmCCR4-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmCCR4-dsRNAres (twin Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    The following variations comprises from our S2 ce…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147271-plasmid-map-sequence-id-289718

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  29. pAc5.1B-EGFP-DmCCR4-dsRNAres_Q

    Plasmid
    #147255
    Purpose
    Insect Expression of DmCCR4-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmCCR4-dsRNAres (twin Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    The following variations comprises from our S2 ce…
    Promoter
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147255-plasmid-map-sequence-id-289702

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  31. pnEA-NvM-DmNot1_1710-2505_P

    Plasmid
    #147188
    Purpose
    Bacterial Expression of DmNot1_1710-2505
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1_1710-2505 (Not1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    three silent and two non silent mutations A1747T …
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147188-plasmid-map-sequence-id-289635

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  32. pAc5.1B-lambdaN-HA-DmNot1-dsRNAres_P

    Plasmid
    #147173
    Purpose
    Insect Expression of DmNot1-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1-dsRNAres (Not1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    10 silent mutations and two non silent N605S, K75…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147173-plasmid-map-sequence-id-289620

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  33. pETM-41-HsNot1iso1_1085-2376_O

    Plasmid
    #147081
    Purpose
    Bacterial Expression of HsNot1iso1_1085-2376
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1_1085-2376 (CNOT1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147081-plasmid-map-sequence-id-289528

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  34. pT7-EGFP-C1-HsDCP1a_1-132_O

    Plasmid
    #147089
    Purpose
    Mammalian Expression of HsDCP1a_1-132
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_1-132 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation T237C compared to the sequenc…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147089-plasmid-map-sequence-id-289536

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  35. pT7-EGFP-C1-HsDCP1a_132-582_O

    Plasmid
    #147090
    Purpose
    Mammalian Expression of HsDCP1a_132-582
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_132-582 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation T1716G compared to the sequen…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147090-plasmid-map-sequence-id-289537

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  36. pETM-41-HsNot1iso1_1085-1605_N

    Plasmid
    #147009
    Purpose
    Bacterial Expression of HsNot1iso1_1085-1605
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1_1085-1605 (CNOT1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    1 silent mutations compared to the sequence of is…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147009-plasmid-map-sequence-id-289456

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  37. pnEA-NvG-DmNot1_1710-2505_N

    Plasmid
    #147019
    Purpose
    Bacterial Expression of DmNot1_1710-2505
    Depositor
    Elisa Izaurralde
    Insert
    DmNot1_1710-2505 (Not1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    three silent and two non silent mutations A1747T …
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147019-plasmid-map-sequence-id-289466

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  38. pLNHA-C1-HsDCP1a_131-582_L

    Plasmid
    #146813
    Purpose
    Mammalian Expression of HsDCP1a_131-582
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_131-582 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one silent mutation T1716G compared to the sequen…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146813-plasmid-map-sequence-id-289260

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  39. pAc5.1B-lambdaN-HA-DmAtx2_J

    Plasmid
    #146624
    Purpose
    Insect Expression of DmAtx2
    Depositor
    Elisa Izaurralde
    Insert
    DmAtx2 (Atx2 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    Three silent mutations and two deletions compared…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146624-plasmid-map-sequence-id-289070

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Showing: 9961 - 10000 of 18127 results