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P1K-3HA-Atg8-MET15

Plasmid

#207032

Purpose

Expression of 3HA-Atg8.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Promoter

pATG8

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCK071

Plasmid

#242907

Purpose

Constitutive expression of dCas9 and PspF-λN22

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

PspF-λN22 (pspF Synthetic)

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_033

Plasmid

#210755

Purpose

PU_Zea mays EF1a promoter

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Zea Mays Elongation factor 1A promoter

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_028

Plasmid

#210759

Purpose

PU_6 x Lac operator sequence 35S minimal fusion promoter (pOp6)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

6 x Lac Operator sequence

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_mEmerald_I3

Plasmid

#231681

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two tandem mEmeralds in mammalian cells. Assembles into nanocages tagged with 120 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I_I3

Plasmid

#239341

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet-I in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mScarlet-I

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_I3

Plasmid

#231680

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with one mEmerald in mammalian cells. Assembles into nanocages tagged with 60 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmStayGold(E138D)_I3

Plasmid

#239338

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mStayGold(E138D)

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+

Plasmid

#232982

Purpose

Galactose iduced expression of Gcn4 Aro2+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Aro2+

Plasmid

#231883

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Expression

Yeast

Promoter

pTEF1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-PGAL-GFP-Cps1

Plasmid

#207030

Purpose

Expression of GFP-Cps1 under GAL promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Cps1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xV5

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xMyc

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

mRuby3

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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