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pN1-HsSmg5-del810-846-V5-SBP-MBP_V

Plasmid

#147765

Purpose

Mammalian Expression of HsSmg5-del810-846

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del810-846 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del847-1017-V5-SBP-MBP_V

Plasmid

#147766

Purpose

Mammalian Expression of HsSmg5-del847-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del847-1017 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7_1-633_U

Plasmid

#147644

Purpose

Mammalian Expression of HsSmg7_1-633

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633 (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G co…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155_T

Plasmid

#147553

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T

Plasmid

#147554

Purpose

Mammalian Expression of HsSmg5-del133-155-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155-G120E (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-Q123E_T

Plasmid

#147555

Purpose

Mammalian Expression of HsSmg5-Q123E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-Q123E (SMG5 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_1-633_T

Plasmid

#147557

Purpose

Mammalian Expression of HsSmg7_1-633

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G compared to the…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNA

Plasmid

#194724

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target tdTomato

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLTR-DuvSAF

Plasmid

#190914

Purpose

Pseudotyping of lentiviral vectors

Depositor

Jakob Reiser

Article

Federici et al Genet Vaccines Ther. 2009 Jan 13;7:1. do

Insert

Lyssavirus duvenhage antigenic glycoprotein

Expression

Mammalian

Promoter

HIV-1 LTR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsSMG7opt_732-1091_V

Plasmid

#147769

Purpose

Bacterial Expression of HsSMG7opt_732-1091

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSMG7opt_732-1091 (SMG7 Human)

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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p3e tdTomato

Plasmid

#188700

Purpose

Gateway 3' element encoding tdTomato

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-G120E_W

Plasmid

#147819

Purpose

Mammalian Expression of HsSmg5-G120E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V

Plasmid

#147767

Purpose

Mammalian Expression of HsSmg5-G120E-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot8_13-240_U

Plasmid

#147649

Purpose

Bacterial Expression of HsNot8_13-240

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8_13-240 (CNOT8 Human)

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

Plasmid

#147558

Purpose

Mammalian Expression of HsSmg7_633-1091

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_633-1091 (SMG7 Human)

Expression

Mammalian

Mutation

one non silent mutation V854I compared to the seq…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-197

Plasmid

#169924

Purpose

WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-197

Expression

Bacterial and Yeast

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-17

Plasmid

#160717

Purpose

TrpC Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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