We narrowed to 7,534 results for: ski

1. pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

   Plasmid

   #101063

   Purpose

   AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   CaMPARI2_F391W-G395D
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO.1-TRC.mKO2_shGFP

   Plasmid

   #110318

   Purpose

   Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX Sap-SH2

   Plasmid

   #46477

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Sap (SH2D1A Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 4-128

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

   Plasmid

   #205442

   Purpose

   production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

   Depositor

   Jeremy Kay

   Article

   Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

   Insert

   Unc5c (Unc5c Mouse)
   

   Use

   AAV

   Tags

   3xHA

   Promoter

   mini-CMV (with MVM small intron)

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MAC-FURIN

   Plasmid

   #158386

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   furin, paired basic amino acid cleaving enzyme (FURIN Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBabe N-Ras 61K

   Plasmid

   #12543

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   NRas 61K (NRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   61K constitutively activated.

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

   Plasmid

   #101064

   Purpose

   AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2_L398T
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIZ-EGFP-MYO10-HMM

   Plasmid

   #87257

   Purpose

   Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYO10 (Myo10 Mouse)
   

   Expression

   Insect

   Mutation

   Myo10 is truncated to contain aa.1-941

   Promoter

   OpIE2

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDH-CMV-NF2-CFP

   Plasmid

   #235687

   Purpose

   Express CFP tagged NF2 gene in mammalian cells using the CMV promoter

   Depositor

   Duojia Pan

   Article

   Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

   Insert

   NF2 (CA12 Human)
   

   Use

   Lentiviral

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.CAG.SF-iGluSnFR.A184S

    Plasmid

    #106198

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.CAG-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106186

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FokI-mCherry-LacI-NLS

    Plasmid

    #215096

    Purpose

    Expresses FokI-mCherry-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    FokI-mCherry-LacI-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX4T3-HA-ERK2 GOF

    Plasmid

    #53200

    Purpose

    Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S/D321N

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBabe H-Ras 12V

    Plasmid

    #12545

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    H-Ras 12V (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    12V constituitively activated.

    Available Since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Marta Gallego, Lothar Hennighausen

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE-hygro-BAP1-HA

    Plasmid

    #154020

    Purpose

    Mammalian Expression of HA-tagged Wild-Type BAP1

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #101061

    Purpose

    AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2-F391W
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139094

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge