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pPN014

Plasmid

#91588

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN082

Plasmid

#91589

Purpose

Express sgRNA targeting human CUL3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CUL3 (CUL3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN083

Plasmid

#91590

Purpose

Express sgRNA targeting human CUL3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CUL3 (CUL3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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CRY-GalVP16 (B695)

Plasmid

#92031

Purpose

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Tags

GalBD-VP16AD

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR (EGFP) shPnky-2

Plasmid

#79142

Purpose

Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

Depositor

Daniel Lim

Article

Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

Insert

shPnky-2 (Gm30731 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for the shRNA and CMV for EGFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_LRRC31

Plasmid

#45271

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

LRRC31 (LRRC31 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
integrin beta6 777T pBS

Plasmid

#13581

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Expression

Bacterial

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-NDUFB5-sgRNA1

Plasmid

#165939

Purpose

CRISPR-mediated gene perturbation (knock-out) of NDUFB5

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

NDUFB5 (NDUFB5 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-NDUFB5-sgRNA2

Plasmid

#165940

Purpose

CRISPR-mediated gene perturbation (knock-out) of NDUFB5

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

NDUFB5 (NDUFB5 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA1

Plasmid

#165943

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA2

Plasmid

#165944

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA1

Plasmid

#165945

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA2

Plasmid

#165946

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA1

Plasmid

#165949

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA2

Plasmid

#165950

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-puro-PFKM

Plasmid

#165953

Purpose

Overexpression of PFKM.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

PFKM (PFKM Human)

Use

Lentiviral

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.A184S

Plasmid

#106174

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1 and AAV9

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pF4Ag NanoLuc (ATG-42)

Plasmid

#137777

Purpose

Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

Depositor

Lance Encell

Article

Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

Insert

NanoLuc (Nluc)

Expression

Bacterial and Mammalian

Mutation

NanoLuc contains 16 amino acid changes compared t…

Promoter

T7 and CMV

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-MUTHY

Plasmid

#215132

Purpose

Expresses mCherry-MUTHY in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MUTHY (MUTYH Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (N154S)

Plasmid

#214160

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (N154S) (STING1 Human)

Expression

Mammalian

Mutation

N154S

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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