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pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

Plasmid

#216247

Purpose

Express OsTIR1.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

OsTIR1.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pF4Ag NanoLuc (ATG-42)

Plasmid

#137777

Purpose

Expresses NanoLuc in multi promoter expression vector (CMV andT7 promoters)

Depositor

Lance Encell

Article

Hall et al ACS Chem Biol. 2012 Nov 16;7(11):1848-57

Insert

NanoLuc (Nluc)

Expression

Bacterial and Mammalian

Mutation

NanoLuc contains 16 amino acid changes compared t…

Promoter

T7 and CMV

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_Cas9–superNLS

Plasmid

#232430

Purpose

Expression plasmid for the SpCas9 nuclease with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cas9–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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TFEB promoter-luciferase reporter

Plasmid

#66801

Purpose

Reporter for activity of TFEB gene promoter

Depositor

Albert La Spada

Article

Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

Insert

TFEB promoter (Tfeb Mouse)

Use

Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#101061

Purpose

AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2-F391W

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgFSP1

Plasmid

#186026

Purpose

knock out FSP1 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Ferroptosis Suppressor Protein 1 (AIFM2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-SNAP

Plasmid

#208773

Purpose

To express C2m2-SNAP in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-SNAP (Mfge8 Mouse)

Tags

SNAP-tag

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-MSN

Plasmid

#210704

Purpose

This plasmid express EF1a promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMarsL274G

Plasmid

#63177

Purpose

This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

MarsL274G (Mars1 Mouse)

Expression

Mammalian

Mutation

L274G

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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Human SHIP1 (clone hp51CN)

Plasmid

#123347

Purpose

Expression of human SHIP1

Depositor

Christophe Erneux

Article

Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

Insert

INPP5D (INPP5D Human)

Expression

Mammalian

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLX307 MCL1

Plasmid

#117726

Purpose

Open reading frame vector encoding MCL1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

MCL1L (MCL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pOT_2 - lenti-EFS-tNGFR-2A-puro

Plasmid

#181971

Purpose

Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

tNGFR (NGFR Human)

Use

Lentiviral

Mutation

truncation: aa 11-276

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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