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pHR-CLIP-CD28(SGGG)

Plasmid

#223607

Purpose

Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

MYPPPY mutated to SGGG

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro 2xStrep-HPC4-Gb5

Plasmid

#218177

Purpose

Used to make a lenti virus for stable expression of the G protein beta 5 subunit in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

G beta subunit 5 (GNB5 Human)

Use

Lentiviral

Tags

2x Strep tag and HPC4

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Uncx [N155/9R]

Plasmid

#114545

Purpose

Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Uncx (Mus musculus) recombinant mouse monoclonal antibody (Uncx Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209061

Purpose

Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

KDM6A

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Uncx [N155/9R]

Plasmid

#206537

Purpose

Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Uncx (Mus musculus) recombinant Mouse monoclonal antibody (Uncx Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC-GST-GINIP

Plasmid

#204844

Purpose

Express GST tagged-GINIP in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

PHF24 (KIAA1045 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1/CALR ins5 (del18-197)-FLAG

Plasmid

#204539

Purpose

Mammalian expression of human CALR ins5 (del18-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del18-197)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del18-197)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del198-430)-FLAG

Plasmid

#204538

Purpose

Mammalian expression of human CALR ins5 (del198-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del198-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del367-430)-FLAG

Plasmid

#204536

Purpose

Mammalian expression of human CALR ins5 (del367-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del367-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del367-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del309-430)-FLAG

Plasmid

#204537

Purpose

Mammalian expression of human CALR ins5 (del309-430)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del309-430)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del309-430)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del1-308)-FLAG

Plasmid

#204535

Purpose

Mammalian expression of human CALR ins5 (del1-308)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-308)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del1-308)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del198-308)-FLAG

Plasmid

#204540

Purpose

Mammalian expression of human CALR ins5 (del198-308)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del198-308)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del198-308)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5 (del1-197)-FLAG

Plasmid

#204534

Purpose

Mammalian expression of human CALR ins5 (del1-197)-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR ins5 (del1-197)-FLAG (CALR Human)

Expression

Mammalian

Mutation

ins5 (del1-197)

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-hCIT

Plasmid

#203166

Purpose

Yeast expression vector for human DHDDS

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

DHDDS (DHDDS Human)

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA1

Plasmid

#201592

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA2

Plasmid

#201593

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-HNRNPD_sgRNA2

Plasmid

#201599

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HNRNPD (HNRNPD Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L_sgRNA1

Plasmid

#201600

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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