We narrowed to 5,601 results for: Feng Zhang

1. pNLF1-C_CCT5:1-541

   Plasmid

   #210979

   Purpose

   Mammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CCT5:M1-E541 (CCT5 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHTC-HaloTag_ACTB:1-375

   Plasmid

   #210971

   Purpose

   Mammalian expression of full-length human ACTB. C-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   ACTB:M1-F375 (ACTB Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pHTC-HaloTag_DCAF12:1-453

   Plasmid

   #210967

   Purpose

   Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   DCAF12:M1-S453 (DCAF12 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   R131Q natural variant (VAR_035322)

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pHTN-HaloTag_ACTB:1-375

   Plasmid

   #210970

   Purpose

   Mammalian expression of full-length human ACTB. N-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   ACTB:M1-F375 (ACTB Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pNLF1-C_DCAF1:1038-1400

   Plasmid

   #210963

   Purpose

   Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   DCAF1:E1038-E1400 (DCAF1 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNLF1-N_CORO1A:1-461

   Plasmid

   #210964

   Purpose

   Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CORO1A:M1-K461 (CORO1A Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNLF1-C_CORO1A:1-461

   Plasmid

   #210965

   Purpose

   Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   CORO1A:M1-K461 (CORO1A Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNLF1-N_DCAF1:1038-1400

   Plasmid

   #210962

   Purpose

   Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   DCAF1:E1038-E1400 (DCAF1 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNLF1-C_FBXW11:1-542

   Plasmid

   #210952

   Purpose

   Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW11:M1-R542 (FBXW11 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBiT3.1-N_SKIC8:1-305

    Plasmid

    #211038

    Purpose

    Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    SKIC8:M1-I305 (SKIC8 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBiT3.1-C_COPB2:1-597

    Plasmid

    #211055

    Purpose

    Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    COPB2:M1-A597 (COPB2 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBiT3.1-N_RBBP4:1-425

    Plasmid

    #211037

    Purpose

    Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    RBBP4:M1-S425 (RBBP4 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pANY6

    Plasmid

    #112198

    Purpose

    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.

    Depositor

    Yingfeng An

    Article

    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

    Type

    Empty backbone

    Use

    Tags

    ELP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pANY3

    Plasmid

    #112197

    Purpose

    This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX458-sgEIF4EBP1

    Plasmid

    #128097

    Purpose

    CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPRv2-sgCTRL

    Plasmid

    #209750

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid

    #113161

    Purpose

    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat Rosa26
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

    Plasmid

    #112719

    Purpose

    An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Mecp2

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiCRISPRv2-sgCD20

    Plasmid

    #209749

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge