We narrowed to 11,231 results for: SHA

1. pAJM.657

   Plasmid

   #108525

   Purpose

   CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   CymRAM + PCymRC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   CymR: S110G A171V

   Promoter

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS423-GPD-wrmScarlet1-10-CYC1 terminator

   Plasmid

   #158584

   Purpose

   Expresses wrmScarlet1-10 in S. cerevisiae

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   wrmScarlet1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

   Plasmid

   #176270

   Purpose

   Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   TadA-TadA*(8e)-dCasMINI-V4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-mStayGold-EFS-Blast

   Plasmid

   #227257

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

   Plasmid

   #199781

   Purpose

   Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

   Depositor

   Sascha Martens

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   GST-Thrombin-TEV-EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-IRES-Neo

   Plasmid

   #85139

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Puro.Cre empty vector

   Plasmid

   #17408

   Purpose

   Lentiviral expression of Cre from the mouse PGK promoter

   Depositor

   Tyler Jacks

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

   Insert

   Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK

   Available since

   March 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-mStayGold-EFS-Zeo

   Plasmid

   #227258

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSico

    Plasmid

    #11578

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG roGFP2-mito

    Plasmid

    #168509

    Purpose

    Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

    Insert

    mito-roGFP2
    

    Use

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOZ-N-FH MERIT40

    Plasmid

    #27498

    Depositor

    Roger Greenberg

    Article

    Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

    Insert

    MERIT40 (BABAM1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pOZ-N-FH KIAA0157

    Plasmid

    #27499

    Depositor

    Roger Greenberg

    Article

    Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

    Insert

    KIAA0157 (ABRAXAS2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

16. AAV-mRuby2-Gephyrin.FingR-IL2RGTC

    Plasmid

    #125696

    Purpose

    Global labeling of inhibitory synapses (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    mRuby2-Gephyrin.FingR-IL2RGTC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAJM.677

    Plasmid

    #108530

    Purpose

    AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    AraCAM + AraE + PBAD-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AraC: L133F E165L E169G C280L

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. ShuttleNSE

    Plasmid

    #50958

    Purpose

    A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter

    Depositor

    James Bamburg

    Article

    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    NSE

    Available since

    Feb. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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