We narrowed to 44,705 results for: cha

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pAV0758

Plasmid

#133511

Purpose

Expression of 3GFP tagged RasAct probe to monitor active Ras1

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)

Expression

Yeast

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0783

Plasmid

#133515

Purpose

Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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VV201: ChR2(C128S)-eGFP in fubi

Plasmid

#58510

Purpose

Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

ChR2(C128S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Cysteine 128 to Serine

Promoter

Ubiquitin

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAV0714

Plasmid

#133479

Purpose

Expression in fission yeast from promoter pact1, integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0750

Plasmid

#133484

Purpose

Expression in fission yeast from promoter purg1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1-GFP

Plasmid

#41615

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP(S65T), TRP1 reverse orientation, and yeast AD…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAV0756

Plasmid

#133508

Purpose

Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0764

Plasmid

#133520

Purpose

Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0773

Plasmid

#133519

Purpose

Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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