We narrowed to 9,135 results for: Pol

1. GAL FLAG72QDProCFPp303

   Plasmid

   #15586

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 72Q Delta Pro (HTT Human)
   

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-pET-RBS

   Plasmid

   #72981

   Purpose

   PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   pET RBS derived from pET vectors
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-ELL2 pBacPAK8

   Plasmid

   #15327

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

   Insert

   Eleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)
   

   Use

   Baculovirus construction

   Tags

   FLAG

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pDONR221 Cas9-RFC4

   Plasmid

   #183199

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC4
   

   Use

   CRISPR; Gateway entry clone

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5B3-T

   Plasmid

   #92021

   Purpose

   Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lysA with N-terminal TEV protease cleavage site
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Mutation

   S219V

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-ATXN1_L588D

   Plasmid

   #48194

   Purpose

   Expresses L588D mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-ATXN1_L588A

   Plasmid

   #48193

   Purpose

   Expresses L588A mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-ATXN1_R665A

   Plasmid

   #48195

   Purpose

   Expresses R665A mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p21 promoter B

    Plasmid

    #16463

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

11. p21 promoter G

    Plasmid

    #16468

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

12. RP78 pUAST Orco mRNA

    Plasmid

    #53431

    Purpose

    Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    Orco (Orco )
    

    Expression

    Insect

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 FYN

    Plasmid

    #67106

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FYN (FYN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. p.UTA.2.0-GL-2

    Plasmid

    #82440

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Mutation

    The complementary region for GL2 siRNA control wa…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRD21A-mRFP

    Plasmid

    #181959

    Purpose

    RD21A protein tagged with mRFP, under control of native promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    RD21A genomic sequence including 2kb upstream of the ATG
    

    Tags

    mRFP

    Expression

    Plant

    Promoter

    RD21A native

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-EAF2 pBacPAK8

    Plasmid

    #15326

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    ELL Associated Factor 2 (EAF2 Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pIS1 DICER1 short UTR

    Plasmid

    #21648

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWL502-PAM

    Plasmid

    #174382

    Purpose

    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    PAM-spacer
    

    Use

    CRISPR; Archaeal expression

    Expression

    Bacterial

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4046938

    Plasmid

    #145599

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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