We narrowed to 10,392 results for: plasmids 101

1. pCRISPR3_BC1

   Plasmid

   #191856

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEL2116

   Plasmid

   #170393

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 1 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ48-159)

   Promoter

   T7

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBEL2117

   Plasmid

   #170394

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 2 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ160-279)

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEL2118

   Plasmid

   #170395

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 3 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ280-391)

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEL2119

   Plasmid

   #170396

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 4 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ392-515)

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEL2120

   Plasmid

   #170397

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 5 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ516-633)

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEL2121

   Plasmid

   #170398

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 6 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ634-746)

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEL2122

   Plasmid

   #170399

   Purpose

   Plasmid for genetic complementation of LetAB with ΔRing 7 LetB mutant

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

   Insert

   LetAB (letB, letA E. coli)
   

   Expression

   Bacterial

   Mutation

   LetA-LetB(Δ747-877)

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC008.106

   Plasmid

   #171141

   Purpose

   Expression of J106 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJF143_SECGFP_sg1

    Plasmid

    #163864

    Purpose

    sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting GFP
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    R07E5.16 (U6)

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC_h7SK M15 (CUA)

    Plasmid

    #154784

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Expression

    Mammalian

    Mutation

    G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYMCR2_Gb_PMLTSS

    Plasmid

    #140285

    Purpose

    CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM136

    Plasmid

    #134177

    Purpose

    Bacterial expression of his-CENP-C 700-943

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

    Insert

    his-CENP-C 700-943 (CENPC Human)
    

    Expression

    Bacterial

    Mutation

    aa 700-943

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV RhoB YFP MS2

    Plasmid

    #131777

    Purpose

    RhoB upstream msfYFP 24xMS2 RhoB downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    CRISPR

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV Btg2 YFP MS2

    Plasmid

    #131776

    Purpose

    Btg2 upstream msfYFP 24xMS2 Btg2 downstream for CRISPR tagging

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    msfYFP - 24x MS2 loops
    

    Use

    CRISPR

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPa-c6MYCn

    Plasmid

    #69242

    Purpose

    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    cMyc

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRP-c6MYCn

    Plasmid

    #69243

    Purpose

    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    cMyc

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRPa-iSL(MCS3+4)

    Plasmid

    #69246

    Purpose

    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Promoter

    RRNA promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA1_pNeurog2-Luciferase reporter

    Plasmid

    #64160

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for pNeurog2-Luciferase reporter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ATLASsnCre

    Plasmid

    #232351

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Has Service

    AAV5

    Insert

    ATLASsnCre
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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