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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCambia2300-Aar1-ccdB-GmCH

Plasmid

#254405

Purpose

Gateway-compatible destination vector for bifluorescence complementation (BiFC). The plasmid carries Gus-mCherry expressed from the Arabidopsis Ubiquitin 10 promoter.

Depositor

Maria Harrison

Article

Ivanov et al Science. 2024 Jan 26;383(6681):443-448.

Type

Empty backbone

Expression

Plant

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_2Ala_APEX2

Plasmid

#250774

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_NUP62-EGFP3

Plasmid

#252748

Purpose

Expression of NUP62 with three tandem EGFPs.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

E3xGFP

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pE-SUMO_PspCas13b

Plasmid

#252295

Purpose

Expresses PspCas13b (WT) in bacterial cells.

Depositor

Hiroshi Nishimasu

Article

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

PspCas13b (WT)

Expression

Bacterial

Mutation

WT

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLV hUBC-stuffer-mCherry-P2A-PuroR

Plasmid

#253231

Purpose

Backbone for insert expression with mCherry-2A-PuroR from lentiviral vector with cloning sites

Depositor

Charles Gersbach

Article

Reisman et al bioRxiv 2025.10.11.681828

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pNORg11-mScarlet-I

Plasmid

#249341

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with mScarlet-I under the control of the constitutive promoter

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Inserts

cymR
veg promoter
CuO
mScarlet-I

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pNORg12-mScarlet-I

Plasmid

#249342

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with mScarlet-I under the control of the cumate-inducible promoter

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Inserts

cymR
fdx promoter
veg promoter
CuO
mScarlet-I

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_mTagBFP2

Plasmid

#250898

Purpose

mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PBAD_R1_EGFP

Plasmid

#250900

Purpose

EGFP expressed under araBAD promoter; part of BNeu AS13; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PBAD

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplA W37V

Plasmid

#248802

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHL_R1_EGFP

Plasmid

#250888

Purpose

EGFP expressed under PHL synthetic promoter ; part of BNeu AS8; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHL

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHT_R4_EGFP

Plasmid

#250889

Purpose

EGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and C55I

Plasmid

#248807

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and C55I

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and R337P

Plasmid

#248805

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and R337P

Plasmid

#248817

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pHK101

Plasmid

#235480

Purpose

Repression of gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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