We narrowed to 33,418 results for: IND

1. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

   Plasmid

   #165083

   Purpose

   gRNA 2 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

   Plasmid

   #165085

   Purpose

   gRNA 1 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. KLC1N343S-Myc

   Plasmid

   #166963

   Purpose

   Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KLC1-N343S (Klc1 Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   KLC1-N343S

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

   Plasmid

   #166020

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Promoter

   CMV51

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPN432

   Plasmid

   #137870

   Purpose

   Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPN431

   Plasmid

   #137869

   Purpose

   Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPN435

   Plasmid

   #137867

   Purpose

   Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPN434

    Plasmid

    #137866

    Purpose

    Expression of gRNA i2 targeting TCF4 for CRISPRi; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPN459

    Plasmid

    #137875

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN446

    Plasmid

    #137868

    Purpose

    Expression of gRNA a1 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPN454

    Plasmid

    #137865

    Purpose

    Expression of gRNA i1 targeting TCF4 for CRISPRi; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid

    #131377

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera DelFSH(307-309)
    

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. EcNGsC-S308P-H309D-pKK223

    Plasmid

    #131378

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308P H309D
    

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EcNGsC-F307A-S308A-pKK223

    Plasmid

    #131379

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera F307A S308A
    

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are fro…

    Promoter

    tac

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCri-H6-TEV-GRAB-STREP

    Plasmid

    #128499

    Purpose

    Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x) and Strep tag (2x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAP1893-1

    Plasmid

    #105248

    Purpose

    human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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