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His-HA-E2p-YFP

Plasmid

#248827

Purpose

Plasmid used to express E2p, a site-specific acceptor protein for LplA, in HEK293T cells. Used to evaluate specific labeling activity of LplA variants.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

E2P

Tags

6xHis-HA and YFP

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and F147S

Plasmid

#248808

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and F147S

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His-LAP-BFP-NLS

Plasmid

#248826

Purpose

LAP2 fused to BFP and NLS, a nuclear localization signal. Imaging shows primarily nucleolar localization.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis and BFP-3xNLS

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and A48N

Plasmid

#248823

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and A48N

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and L172A

Plasmid

#248815

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and L172A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V

Plasmid

#248814

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA

Plasmid

#248813

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplA Wild Type

Plasmid

#248801

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and L172A

Plasmid

#248803

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and L172A

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3HA::tbTurboID::3HA

Plasmid

#247590

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tbTurboID

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3HA::tbTurboID::3HA

Plasmid

#247592

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tbTurboID

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-3HA

Plasmid

#247594

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3HA::FRB::3HA

Plasmid

#247587

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3HA::tbTurboID::3HA

Plasmid

#247591

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tbTurboID

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3HA::FRB::3HA

Plasmid

#247589

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF7.5-Dadh-ΔElen0475(ElA2)

Plasmid

#225320

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0475) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0475)

Tags

His8

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF2-Elen0501-(+)-Eddh-Accs(ElA2)

Plasmid

#225297

Purpose

Expresses Eggerthella lenta A2 (+)-Eddh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_KO_Puro_donor

Plasmid

#246401

Purpose

PHF19 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_KO_Puro_donor

Plasmid

#246393

Purpose

MTF2 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYSGDLuc

Plasmid

#236297

Purpose

Dual luciferase reporter for measuring recoding efficiencies in yeast

Depositor

Pasha Baranov

Article

Fenton et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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