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Plasmid#27003DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationS382A S468APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA, mitfa:Cas9
Plasmid#118840PurposeTargets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-μ1A-(HA)3
Plasmid#198177PurposeExpression of HA-tagged AP-1 μ1A in mammalian cellsDepositorInsertAP-1 μ1A
UseTagstriple HA tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM2-FRB-CFP
Plasmid#67518PurposeFRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)DepositorInsertmTOR (MTOR Human)
UseTagsCFP, HA, and PM tethering sequence from human Lyn…ExpressionMammalianMutationFRB domain only (residues 2019–2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Consensus
Plasmid#176664PurposeExpression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Nd1
Plasmid#191523PurposeExpression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vectorDepositorInsertv8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKH162_FLAG-APEX2-YIPF3 LIR mut
Plasmid#214981PurposeFlag-APEX2 tagged LIR mutant YIPF3DepositorInsertFLAG-APEX2-YIPF3_mut (YIPF3 Human)
UseLentiviralTagsExpressionMutationLIR mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_peroxisome_C1
Plasmid#85064PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
UseTagsmScarlet-HExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131A2.0
Plasmid#99884PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-Smad3 WT
Plasmid#27010DepositorInsertSmad3 (SMAD3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pZ001 (AacC2c1 locus)
Plasmid#70166PurposeExpresses AacC2c1 full locusDepositorInsertAacC2c1 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Agam_695
Plasmid#176654PurposeExpression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG4-CMV-luc
Plasmid#46322DepositorInsert5xGal4
UseLuciferaseTagsCMV promoter and luciferaseExpressionMammalianMutationfive copies of a multimerized GAL4 (5×GAL4) site …PromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Aaeg_774
Plasmid#176659PurposeExpression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro Brca1 S1423A S1524A HA
Plasmid#41968DepositorInsertBRCA1 (BRCA1 Human)
UseRetroviralTagsHAExpressionMammalianMutationS1423A, S1524APromoterAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLN599 (pFuGW-hUbCp-hSTE1)
Plasmid#105206Purposeubiquitin C promoter drives STE expressionDepositorInserthUbCp-hSTE1
UseLentiviral and Synthetic BiologyTagsHA tagExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX303 GFP-PIK3C2A
Plasmid#162000PurposeLentiviral expression vector containing PIK3C2A tagged with GFPDepositorInsertPIK3C2A (PIK3C2A Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN379 (FuGW-S(E2F1)p-mKate2)
Plasmid#105177Purposesynthetic promoter E2F1 drives mKate2 expressionDepositorInsertS(E2F1)p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH2217
Plasmid#179251PurposePsra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherryDepositorInsertTWK-18 (twk-18 Nematode)
UseTagswCherryExpressionWormMutationM280IPromoterPsra-11Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only