We narrowed to 143,240 results for: ins

1. pCAG-ChRmine-mRuby2-ST

   Plasmid

   #227777

   Purpose

   Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChRmine-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASTattB-dALFANb(5mut)-mGreenLantern

   Plasmid

   #219661

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASTattB-dALFANb(3mut)-mGreenLantern

   Plasmid

   #219662

   Purpose

   Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK331

   Plasmid

   #59572

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(16) 2xK-turn Kozak EGFP attB2 AscI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTH PKC alpha

   Plasmid

   #8409

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Mouse Protein Kinase C alpha (Prkca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CAG-HcKCR2-EYFP

   Plasmid

   #182022

   Purpose

   Express light-gated potassium channel HcKCR2-EYFP in mammalian cells

   Depositor

   Mingshan Xue

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-hDicer

   Plasmid

   #89144

   Purpose

   Expression of hDicer in Sf9 Cells

   Depositor

   Jennifer Doudna

   Article

   MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

   Insert

   hDicer (DICER1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167942

   Purpose

   Inducible NKX2-1 overexpression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   TTF1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   tetON

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-p25

   Plasmid

   #24896

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

   Insert

   p25 (CDK5R1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal truncated version of p35, contains aa …

   Promoter

   Available since

   May 17, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pBABE puro mouse IRS-2 myc

    Plasmid

    #11373

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. HB501: pMVP (L1-L4) RIP

    Plasmid

    #121688

    Purpose

    pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Rat Ins1 promoter (RIP; β-cell specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX302 OAS2-V5 puro

    Plasmid

    #158642

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS2 (OAS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis-hMTMR1

    Plasmid

    #100723

    Purpose

    Expression of human MTMR1 PH-PTP domain

    Depositor

    Byung Il Lee

    Article

    Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

    Insert

    MTMR1 (MTMR1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 95-607

    Promoter

    T7

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19 PKC delta

    Plasmid

    #8427

    Depositor

    Frederic Mushinski

    Article

    Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

    Insert

    Mouse Protein Kinase C delta (Prkcd Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEM272 - MCS mosTI Pmlc-2_GFP target

    Plasmid

    #159834

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI Pmlc-2_GFP target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEM255 - sgRNA mosTI Pmlc-2_GFP

    Plasmid

    #159835

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI Pmlc-2_GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge