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pET30b GluBP600n

Plasmid

#119835

Purpose

Production of recombinant GluBP600n high affinity protein in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

GluBP600n

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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gRD21A-mRFP

Plasmid

#181959

Purpose

RD21A protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

RD21A genomic sequence including 2kb upstream of the ATG

Tags

mRFP

Expression

Plant

Promoter

RD21A native

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET41a iGlul

Plasmid

#119832

Purpose

Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlul

Tags

GST

Expression

Bacterial

Mutation

T92A

Promoter

T7

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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LNPL1093-piggybac-Dox-inducible-CasRx-QK634-UnirapR-IRES-mCherry

Plasmid

#248111

Purpose

Expresses dox-inducible ChemoCas13d or CasRx-QK634-LightR (UnirapR domain inserted right after amino acid residue 634 of CasRx)

Depositor

Jared Toettcher

Article

Zhu et al bioRxiv 2025.04.21.649818

Insert

CasRx-UnirapR

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

Tet inducible

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHD Btl-pYtag(Scarlet) ScarlessDsRed

Plasmid

#244937

Purpose

Vector for tagging Breathless (Btl) with Scarlet-based pYtag biosensor. DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

Endogenous tagging

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Torso

Plasmid

#244933

Purpose

CRISPR gRNA targeting the C-terminal end of Torso for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Torso C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-Btl

Plasmid

#244934

Purpose

CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting Btl C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-EGFR

Plasmid

#244932

Purpose

CRISPR gRNA targeting the C-terminal end of EGFR for cleavage.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

gRNA targeting EGFR C terminus

Use

CRISPR

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MZB215

Plasmid

#221767

Purpose

Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf6 (ARF6 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q67L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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