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We narrowed to 4,045 results for: golden gate

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Showing: 1251 - 1300 of 4045 results

  1. pKS1601

    Plasmid
    #239526
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239526-plasmid-map-sequence-id-475905

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  2. pKS1531

    Plasmid
    #239521
    Purpose
    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239521-plasmid-map-sequence-id-475896

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  3. pKS1575

    Plasmid
    #239523
    Purpose
    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239523-plasmid-map-sequence-id-475887

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  4. pVL1337

    Plasmid
    #239541
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-ScPex15(315-383)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239541-plasmid-map-sequence-id-475879

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  5. pKS1574

    Plasmid
    #239522
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScYea4p-mTurquoise2
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239522-plasmid-map-sequence-id-475877

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  6. pAIP4_3W4Q

    Plasmid
    #234236
    Purpose
    Heterologous protein expression of beta-lactamase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3W4Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234236-plasmid-map-sequence-id-464200

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  7. pAIP4_3ILS

    Plasmid
    #234215
    Purpose
    Heterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ILS
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234215-plasmid-map-sequence-id-464180

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  8. pAIP4_5NJO

    Plasmid
    #234202
    Purpose
    Heterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5NJO
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234202-plasmid-map-sequence-id-464167

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  9. pAIP4_1ZHV

    Plasmid
    #234197
    Purpose
    Heterologous protein expression of aspartate kinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1ZHV
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234197-plasmid-map-sequence-id-464162

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  10. pAIP4_1NG6

    Plasmid
    #234191
    Purpose
    Heterologous protein expression of YqeY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1NG6 (yqeY Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234191-plasmid-map-sequence-id-464156

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  11. pAIP4_4QB4

    Plasmid
    #234187
    Purpose
    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QB4
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234187-plasmid-map-sequence-id-464152

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  12. pAIP4_2OHW

    Plasmid
    #234178
    Purpose
    Heterologous protein expression of YueI in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OHW (yueI Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234178-plasmid-map-sequence-id-464143

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  13. pAIP4_4CFI

    Plasmid
    #234163
    Purpose
    Heterologous protein expression of flagellin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4CFI (QUF21_RS17235 Burkholderia pseudomallei K96243)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234163-plasmid-map-sequence-id-464129

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  14. L1P139 - pSPCH-PhiC31-tUBQ1

    Plasmid
    #219703
    Purpose
    Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH:PhiC31:tUBQ1
    Expression
    Plant
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219703-plasmid-map-sequence-id-435733

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  15. L0-T15 BpiI-p35s-BpiI

    Plasmid
    #219698
    Purpose
    Level 0 to insert p35S promoter to history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    BpiI-p35s-BpiI
    Use
    Synthetic Biology
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219698-plasmid-map-sequence-id-435729

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  16. pBD026

    Plasmid
    #201309
    Purpose
    AvrSr27-2 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-2
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201309-plasmid-map-sequence-id-410982

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  17. pBD025

    Plasmid
    #201308
    Purpose
    AvrSr27-1 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-1
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201308-plasmid-map-sequence-id-409510

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  18. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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  19. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  20. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  21. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

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  22. T15m

    Plasmid
    #121034
    Purpose
    MoClo golden gate assembly DE part for L3S3P11 (173x-strength synthetic terminator, modified to remove BsaI cut site; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S3P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121034-plasmid-map-sequence-id-245426

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  23. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  24. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  25. C82m

    Plasmid
    #120993
    Purpose
    MoClo golden gate assembly CD part for RpaI (synthase for Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine lactone). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120993-plasmid-map-sequence-id-236098

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  26. C38m

    Plasmid
    #120971
    Purpose
    MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 38 (RpoS)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120971-plasmid-map-sequence-id-236023

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  27. P60m

    Plasmid
    #120952
    Purpose
    MoClo golden gate assembly AB for pSalTTTC (Adam Meyer's improved pSal promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pSal Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120952-plasmid-map-sequence-id-235966

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  28. C1m

    Plasmid
    #120955
    Purpose
    MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)
    Depositor
    Richard Murray
    Insert
    deGFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120955-plasmid-map-sequence-id-235968

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  29. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

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  30. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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  31. C22m

    Plasmid
    #120963
    Purpose
    MoClo golden gate assembly CD part for mSpinach (fluorescent RNA aptamer for DFHBI, suitable for in vivo use; see DOI: 10.1021/sb400089c). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mSpinach
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120963-plasmid-map-sequence-id-236357

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  32. P37m

    Plasmid
    #120947
    Purpose
    MoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pRpa* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120947-plasmid-map-sequence-id-236378

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  33. P38m

    Plasmid
    #120948
    Purpose
    MoClo golden gate assembly AB part for pTra* (improved pTra from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pTra* from Scott and Hasty 2016
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120948-plasmid-map-sequence-id-236376

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  34. P58m

    Plasmid
    #120950
    Purpose
    MoClo golden gate assembly AB for pLas-A-10b (Adam Meyer's improved pLas promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLas Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120950-plasmid-map-sequence-id-235962

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  35. P59m

    Plasmid
    #120951
    Purpose
    MoClo golden gate assembly AB part for pLux76B (Adam Meyer's improved pLux promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    pLux Adam Meyer
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120951-plasmid-map-sequence-id-236155

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  36. p3m

    Plasmid
    #120935
    Purpose
    MoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23150
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120935-plasmid-map-sequence-id-235944

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  37. p4m

    Plasmid
    #120936
    Purpose
    MoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    J23151
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120936-plasmid-map-sequence-id-235945

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  38. pAIP4_9C74

    Plasmid
    #234238
    Purpose
    Heterologous protein expression of variant of super folded GFP in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    9C74
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234238-plasmid-map-sequence-id-464202

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  39. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199652-plasmid-map-sequence-id-412250

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  40. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  41. T16m

    Plasmid
    #121035
    Purpose
    MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P55 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121035-plasmid-map-sequence-id-245903

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  42. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

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  43. T12m

    Plasmid
    #121031
    Purpose
    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120029600 (strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121031-plasmid-map-sequence-id-249041

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  44. RcP9

    Plasmid
    #242157
    Purpose
    Recombineering Plasmid. ITPG-induced expression of Recombitron operon (ccdB dropout cassette in Eco1 Retron); PSP-Tet promoter for Gene III induction upon M13 phage infection and aTc addition.
    Depositor
    Dominik Niopek
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242157-plasmid-map-sequence-id-494263

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  45. pKS1603

    Plasmid
    #239527
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239527-plasmid-map-sequence-id-479035

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  46. pKS1616

    Plasmid
    #239547
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239547-plasmid-map-sequence-id-479031

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  47. pKS1615

    Plasmid
    #239546
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239546-plasmid-map-sequence-id-478363

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  48. pLII-CRISPR for CKA4

    Plasmid
    #238521
    Purpose
    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha4 (AT2G23070 Mustard Weed)
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238521-plasmid-map-sequence-id-477173

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  49. pKS1613

    Plasmid
    #239531
    Purpose
    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)-SKL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239531-plasmid-map-sequence-id-475886

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  50. pVL1281

    Plasmid
    #239540
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239540-plasmid-map-sequence-id-475863

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