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We narrowed to 5,049 results for: golden gate

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Showing: 1251 - 1300 of 5049 results

  3. pAV10.K3.loxP

    Plasmid
    #63202
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63202-plasmid-map-sequence-id-104899

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  4. pAV10.HO6

    Plasmid
    #63211
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63211-plasmid-map-sequence-id-115802

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  5. AMBERv1 (pICSL80187)

    Plasmid
    #252515
    Purpose
    Level 0 plasmid containing AMBERv1 CDS (pICSL80187)
    Depositor
    Sophien Kamoun
    Insert
    AMBERv1
    Expression
    Plant
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252515-plasmid-map-sequence-id-504963

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  6. AMBERv2 (pICSL80189)

    Plasmid
    #252516
    Purpose
    Level 0 plasmid containing AMBERv2 CDS (pICSL80189)
    Depositor
    Sophien Kamoun
    Insert
    AMBERv2
    Expression
    Plant
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252516-plasmid-map-sequence-id-504964

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  7. GOLD (pICSL80188)

    Plasmid
    #252517
    Purpose
    Level 0 plasmid containing GOLD CDS (pICSL80188)
    Depositor
    Sophien Kamoun
    Insert
    GOLD
    Expression
    Plant
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252517-plasmid-map-sequence-id-504961

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  8. C9m

    Plasmid
    #120958
    Purpose
    MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    deCFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120958-plasmid-map-sequence-id-235973

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  9. C76m

    Plasmid
    #120988
    Purpose
    MoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    KanR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120988-plasmid-map-sequence-id-236053

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  10. RUBY (pICSL80094)

    Plasmid
    #252519
    Purpose
    Level 0 plasmid containing RUBY CDS (pICSL80094)
    Depositor
    Sophien Kamoun
    Insert
    RUBY
    Expression
    Plant
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252519-plasmid-map-sequence-id-504962

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  11. pBD_PYR1_156-F1

    Plasmid
    #241286
    Purpose
    Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;
    Depositor
    Sean Cutler
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    241286-plasmid-map-sequence-id-484590

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  12. pPSG804

    Plasmid
    #239534
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YDR514C
    Use
    CRISPR
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239534-plasmid-map-sequence-id-479034

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  13. pPSG779

    Plasmid
    #239532
    Purpose
    CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YBR197C
    Use
    CRISPR
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239532-plasmid-map-sequence-id-478373

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  14. pKS1469

    Plasmid
    #239517
    Purpose
    Express the upper portion of the melvaonate pathway enzymes (MvaE and MvaS) in the peroxisome
    Depositor
    John Dueber
    Insert
    EfMvaE-PTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239517-plasmid-map-sequence-id-475898

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  15. pPSG803

    Plasmid
    #239533
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YMR206W
    Use
    CRISPR
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239533-plasmid-map-sequence-id-475867

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  16. pAIP4_1EDG

    Plasmid
    #234144
    Purpose
    Heterologous protein expression of GUNA_RUMCH in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EDG
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234144-plasmid-map-sequence-id-464110

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  17. U4m

    Plasmid
    #121017
    Purpose
    MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121017-plasmid-map-sequence-id-236179

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  18. PT7-alphaHL

    Plasmid
    #227650
    Purpose
    pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.
    Depositor
    Richard Murray
    Insert
    alpha-hemolysin
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227650-plasmid-map-sequence-id-452398

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  19. pAIP4_1UXZ

    Plasmid
    #234166
    Purpose
    Heterologous protein expression of cellulase B in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UXZ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234166-plasmid-map-sequence-id-464132

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  20. pHIE096 CMV Bxb1

    Plasmid
    #139252
    Purpose
    Encodes a Bxb1 recombinase for Landing Pad integration.
    Depositor
    Joshua Leonard
    Insert
    Bxb1 Recombinase
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139252-plasmid-map-sequence-id-325167

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  21. pPD1019 TRE3GV DsRedExpress2 in TU2

    Plasmid
    #139254
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV2
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139254-plasmid-map-sequence-id-325170

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  22. pJM467 TRE3GV EYFP in TU3

    Plasmid
    #139255
    Purpose
    TRE3GV promoter and EYFP in TUPV3
    Depositor
    Joshua Leonard
    Insert
    EYFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139255-plasmid-map-sequence-id-325172

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  23. pKS1466

    Plasmid
    #239516
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239516-plasmid-map-sequence-id-475876

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  24. pKS1137

    Plasmid
    #239498
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239498-plasmid-map-sequence-id-475872

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  25. pKS1169

    Plasmid
    #239503
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239503-plasmid-map-sequence-id-475865

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  26. pKS1025

    Plasmid
    #239496
    Purpose
    Express inactive peroxisomal RsAS as negative control
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    Expression
    Yeast
    Mutation
    H360E
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239496-plasmid-map-sequence-id-475861

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  27. pAIP4_3S60

    Plasmid
    #234231
    Purpose
    Heterologous protein expression of lectin OAA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3S60
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234231-plasmid-map-sequence-id-464330

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  28. pAIP4_5M2Y

    Plasmid
    #234193
    Purpose
    Heterologous protein expression of B7LG64_ECO55 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5M2Y
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234193-plasmid-map-sequence-id-464158

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  29. pAIP4_3H6Q

    Plasmid
    #234185
    Purpose
    Heterologous protein expression of macrocypin-1a in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3H6Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234185-plasmid-map-sequence-id-464150

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  30. pAIP4_4Q2S

    Plasmid
    #234177
    Purpose
    Heterologous protein expression of C20G4.08 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4Q2S
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234177-plasmid-map-sequence-id-464142

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  31. pAIP4_4O6G

    Plasmid
    #234175
    Purpose
    Heterologous protein expression of uncharacterized protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4O6G
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234175-plasmid-map-sequence-id-464140

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  32. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

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  33. pBD006

    Plasmid
    #201306
    Purpose
    D16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D16: Promoter of TraesCS5D02G320700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201306-plasmid-map-sequence-id-409554

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  34. pBD004

    Plasmid
    #201304
    Purpose
    D14: Promoter of TraesCS3D02G368800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D14: Promoter of TraesCS3D02G368800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    201304-plasmid-map-sequence-id-409541

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  35. pBD005

    Plasmid
    #201305
    Purpose
    D15: Promoter of TraesCS3D02G517500 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D15: Promoter of TraesCS3D02G517500
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201305-plasmid-map-sequence-id-409522

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  36. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid
    #139257
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV5
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139257-plasmid-map-sequence-id-325370

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  37. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

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  38. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

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  39. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

    Enlarge

  40. pAV10.F6.loxP

    Plasmid
    #63201
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Oct. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    63201-plasmid-map-sequence-id-267056

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  43. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

    Enlarge

  45. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

    Enlarge

  46. pAV10.K6.loxP

    Plasmid
    #63203
    Purpose
    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63203-plasmid-map-sequence-id-104900

    Enlarge

  47. pAV10.K5.loxP

    Plasmid
    #63204
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63204-plasmid-map-sequence-id-104903

    Enlarge

  48. pAV10.HO3.loxP

    Plasmid
    #63205
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63205-plasmid-map-sequence-id-104905

    Enlarge

  49. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

    Enlarge

  50. pET26b-BsaI

    Plasmid
    #233523
    Purpose
    Purification vector with BsaI cut sites for streamlined cloning
    Depositor
    Carl Denard
    Type
    Empty backbone
    Tags
    6xHis-tag and pelB leader
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233523-plasmid-map-sequence-id-474147

    Enlarge

Showing: 1251 - 1300 of 5049 results