Addgene

We narrowed to 823 results for: 3xha

Showing: 501 - 520 of 823 results

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6xHis-MBP-huPrCas12a

Plasmid

#174674

Purpose

Expresses 6xHis-MBP tagged PrCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPrCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBfCas12a

Plasmid

#174672

Purpose

Expresses 6xHis-MBP tagged BfCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBfCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Flu cTbx4 (CT#700)

Plasmid

#13925

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx4 (TBX4 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pFA6a-3HA-TRP1

Plasmid

#41599

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA and yeast ADH1 terminator

Expression

Mutation

Promoter

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti UBC hNLRP3-mNG

Plasmid

#218642

Purpose

Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3HA

Plasmid

#221048

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA epitopes

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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HA-GFP_rescue_construct

Plasmid

#190641

Purpose

Puromycin-selectable expression of GFP in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

EGFP

Use

Tags

3xHA

Expression

Insect

Mutation

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+HA-hSeparase

Plasmid

#33018

Depositor

Marc Kirschner

Article

Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

Insert

Separase (ESPL1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pIE2-DBD

Plasmid

#21602

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 May 26. ():.

Insert

GAL4 DBD (GAL4 Budding Yeast)

Use

Gateway destination vector

Tags

3xHA and Gateway destination cassette

Expression

Insect

Mutation

Promoter

Available since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Flu cPitx2 (CT#704)

Plasmid

#13928

Depositor

Cliff Tabin

Article

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
p3HM2Luc

Plasmid

#234807

Purpose

Mammalian expression of human muscarinic M2 receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

Muscarinic M2 receptor

Use

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3HB2ALuc

Plasmid

#234808

Purpose

Mammalian expression of human B2-adrenergic receptor fused to RLuc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

B2-adrenergic receptor

Use

Tags

3xHA and RLuc8

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124IHA

Plasmid

#232993

Purpose

Galactose iduced expression of Gcn4 F124IHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 F124I

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WTSLFD

Plasmid

#232980

Purpose

Galactose iduced expression of Gcn4 WTSLFD+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WTSLFD+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AHA

Plasmid

#232992

Purpose

Galactose iduced expression of Gcn4 W120AHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 W120A

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Aro3+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WT 44mer

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Aro2+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WW+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 R++ W+

Use

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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