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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,218 results for: Coli

Showing: 501 - 520 of 9218 results

  1. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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  2. pInt_attP1_sacB_kanR

    Plasmid
    #205301
    Purpose
    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205301-plasmid-map-sequence-id-404791

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  3. pInt_attP1_kanR

    Plasmid
    #205294
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205294-plasmid-map-sequence-id-407001

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  4. pInt_attP2_kanR

    Plasmid
    #205297
    Purpose
    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#2).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205297-plasmid-map-sequence-id-415623

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  5. pInt_attP3_chlorR

    Plasmid
    #205298
    Purpose
    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205298-plasmid-map-sequence-id-407000

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  6. pInt_attP5_ampR

    Plasmid
    #205299
    Purpose
    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#5).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205299-plasmid-map-sequence-id-406998

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  7. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  8. pJK645

    Plasmid
    #71700
    Purpose
    Produces E. coli thioredoxin (EcTrxA)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71700-plasmid-map-sequence-id-131737

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  9. pInt_attP1_chlorR

    Plasmid
    #205295
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205295-plasmid-map-sequence-id-406999

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  10. pInt_attP1_ampR

    Plasmid
    #205296
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205296-plasmid-map-sequence-id-406997

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  11. pJK647

    Plasmid
    #71701
    Purpose
    Produces E. coli thioredoxin reductase (EcTrxB)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71701-plasmid-map-sequence-id-131729

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  12. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid
    #177209
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177209-plasmid-map-sequence-id-348605

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  13. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  14. Ec holD-pET(3c)

    Plasmid
    #237236
    Purpose
    over-express E. coli holD (Pol III psi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holD (psi) (holD E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237236-plasmid-map-sequence-id-469263

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  15. Ec dnaN-pET(11a)

    Plasmid
    #237238
    Purpose
    over-express E. coli dnaN (Pol III beta sliding clamp subunit)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta) (dnaN E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237238-plasmid-map-sequence-id-469258

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  16. Ec holA-pET(11a)(ACH-)

    Plasmid
    #237233
    Purpose
    over-express E. coli holA (Pol III delta subunit)
    Depositor
    Michael O'Donnell
    Insert
    holA (delta) (holA E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237233-plasmid-map-sequence-id-469254

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  17. Ec holC-pET(3c)

    Plasmid
    #237235
    Purpose
    over-express E. coli holC (Pol III chi subunit)
    Depositor
    Michael O'Donnell
    Insert
    holC (chi) (holC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237235-plasmid-map-sequence-id-469249

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  18. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  19. p416-GPD-HA-PPK(mutant)

    Plasmid
    #183944
    Purpose
    Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183944-plasmid-map-sequence-id-363303

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  20. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    177208-plasmid-map-sequence-id-356946

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Showing: 501 - 520 of 9218 results