We narrowed to 10,893 results for: ENA;
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Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQF-mNGsacB
Plasmid#203673PurposepQF with mNeonGreen and sacB under control of PQ5DepositorInsertsmNeonGreen
sacB
UseTagsExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQ5Available SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-SaDAH
Plasmid#140128PurposeExpresses SaDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Sinomenium acutum
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQFT-lacZ
Plasmid#208896PurposepQFT with lacZ under control of PQJDepositorInsertlacZ
UseTagsExpressionBacterialMutationContains two silent mutations and a missense muta…PromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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NCVB-ssGrl1-ESCargo*
Plasmid#140167PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophilaDepositorInsertssGrl1-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBUN279
Plasmid#60674Purposeintegration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB siteDepositorInsertald
UseE.coli-mycobacterium integration-proficient vectorTagsExpressionMutationPromoterAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GST
Plasmid#134592PurposeLow Phosphate inducible GSTDepositorInsertGlutathione S-transferase
UseTagsnoneExpressionBacterialMutationPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSgk1_v3 Flag
Plasmid#86039Purposefull length human Sgk1_i3 with Flag tagDepositorInsertAlternate transcript v3 of human Sgk1 (SGK1 Human)
UseTagsFLAGExpressionMammalianMutationv3; S313GPromoterCMVAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Cav (1-61)
Plasmid#22446DepositorInsertCaveolin1 (1-61)
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceJan. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDF-yibDp-matB
Plasmid#134597PurposeLow Phosphate inducible matB (malonyl-CoA synthetase)DepositorInsertMalonyl CoA synthetase
UseTagsExpressionBacterialMutationPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PA1024_pET21a(+)C-tag
Plasmid#14579DepositorInsert2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)
Plasmid#225438PurposeTranscriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.DepositorInsertCBS-FRT-OCS-BeYDVRepRepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)
Plasmid#225436PurposeBeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.DepositorInsertBeYDV geminino 1.0
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)
Plasmid#225430PurposeOCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoterDepositorInsertminiDFR-FRT-OCSterm-FRT-miniDFR
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
JGI_018311
Plasmid#158833PurposeRare earth element alcohol dehydrogenasesDepositorInsertFAM1_3631
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018387
Plasmid#158871PurposeRare earth element alcohol dehydrogenasesDepositorInsertMethyloceanibacter_5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018349
Plasmid#158852PurposeRare earth element alcohol dehydrogenasesDepositorInsertLP2A_1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGI_018361
Plasmid#158858PurposeRare earth element alcohol dehydrogenasesDepositorInsertDokdonella_3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only