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We narrowed to 1,118 results for: gibson assembly

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Showing: 501 - 520 of 1118 results

  3. pJB75

    Plasmid
    #226320
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Publication
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis-(Y to A MR1-6)-strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226320-plasmid-map-sequence-id-449984

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  4. pJB65

    Plasmid
    #226319
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Publication
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis- (VTG to AAA MR1-6)-strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226319-plasmid-map-sequence-id-449981

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  5. pQE80L TriCatcher-RGDSP-MMP

    Plasmid
    #112633
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Publication
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-RGDSP-MMP
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112633-plasmid-map-sequence-id-222151

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  6. pEND-320_Cacnes_ori

    Plasmid
    #225602
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Publication
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225602-plasmid-map-sequence-id-451377

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  17. p005-RFP-weak

    Plasmid
    #108316
    Purpose
    Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Publication
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    RFP under controlf of weak synthetic constitutive promoter pFAB4024
    Expression
    Bacterial
    Promoter
    pFAB4024
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108316-plasmid-map-sequence-id-218822

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  19. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

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  20. Integrin β1β6-EGFP

    Plasmid
    #242826
    Purpose
    Encodes human integrin β1 chimera containing integrin β6 cytoplasmic domain and C-terminal EGFP tag
    Depositor
    Cheng-han Yu
    Publication
    Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi
    Insert
    Integrin subunit beta 1, transcript variant 1A (ITGB1 Human)
    Expression
    Mammalian
    Mutation
    Integrin β1 chimera containing the cytoplasmic ta…
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242826-plasmid-map-sequence-id-486257

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Showing: 501 - 520 of 1118 results