We narrowed to 3,225 results for: zebrafish

1. 240 pTol2005b

   Plasmid

   #73483

   Purpose

   Tol2 vector with multi cloning site

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Type

   Empty backbone

   Use

   Tol2

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. 362 pCS Cherry DEST

   Plasmid

   #13075

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   Cherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. 223 pCS EGFP DEST

   Plasmid

   #13071

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. 221 pCS 3MT DEST

   Plasmid

   #13070

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   6x Myc Tag
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 18, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTRbasEGFP

   Plasmid

   #22453

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   EGFP (eGFP )
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3Eegfp

   Plasmid

   #22462

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   EGFP (eGFP )
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTRegfp3

   Plasmid

   #22464

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   EGFP (eGFP )
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

   Plasmid

   #192714

   Purpose

   Mammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag

   Depositor

   Summer Thyme

   Insert

   MCP-ADAR2-DD(E488Q/T490A)-V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   E488Q/T490A

   Promoter

   CMV

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. actc1b-mKate2-P2A-APEX2-VHH[GFP]

   Plasmid

   #126749

   Purpose

   Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   VHH[EGFP]
   

   Use

   Zebrafish muscle expression clone

   Tags

   mKate2-P2A-APEX2

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

    Plasmid

    #108871

    Purpose

    Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Inserts

    5xU6:sgRNA
    Cas9-t2A-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and hsp70

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-nCas9n-nanos 3’UTR

    Plasmid

    #62542

    Purpose

    expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

    Insert

    nanos 3' UTR (nanos3 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    3' UTR only

    Promoter

    SP6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid

    #78250

    Purpose

    Can be used to subclone in gene of interest into blue motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    

    Use

    Zebrafish motor neuron expression

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDestTol2-actinb-kcna1a-IRES-EGFP

    Plasmid

    #164965

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcna1a (kcna1a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-Lifeact-mRuby

    Plasmid

    #194339

    Purpose

    Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Lifeact
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-GFP1-9

    Plasmid

    #124433

    Purpose

    Expresses GFP1-9 in zebrafish

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS-zAsCpf1

    Plasmid

    #107185

    Purpose

    plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mutation

    Promoter

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+PhyBΔCreC

    Plasmid

    #131781

    Purpose

    Mammalian, Zebrafish, or Xenopus expression of PhyBΔCreC, one half of the CreLite system.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-mRuby-Rab8a

    Plasmid

    #194337

    Purpose

    Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Rab8 (RAB8A Canis lupus)
    

    Use

    Tags

    mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

    Plasmid

    #67668

    Purpose

    Zebrafish expression vector for ubiquitous expression of APEX2-GBP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Zebrafish expression with tol2

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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