We narrowed to 2,931 results for: PPL

1. C39m

   Plasmid

   #120972

   Purpose

   MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ccdB toxin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site removed

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-gR-PnTW

   Plasmid

   #133356

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

   Depositor

   Blair Madison

   Insert

   tdTomato-NLS
   

   Use

   Tags

   HA-tagged tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. C67m

   Plasmid

   #120981

   Purpose

   MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 integrase tagged with an mScarlet-I reporter and an LAA degradation tag). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Bxb1 integrase - mScarlet-i - LAA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. UC16m

   Plasmid

   #121038

   Purpose

   MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gQi Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. UC17m

   Plasmid

   #121039

   Purpose

   MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   gV1 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. C105m

   Plasmid

   #121008

   Purpose

   MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP-AAV ssrA (has bbsI)
   

   Use

   Synthetic Biology

   Tags

   AAV degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C106m

   Plasmid

   #121009

   Purpose

   MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP-DAS ssrA (has bbsI)
   

   Use

   Synthetic Biology

   Tags

   DAS degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. C109m

   Plasmid

   #121011

   Purpose

   MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mfLon protease with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXR003_puroR

   Plasmid

   #219819

   Purpose

   hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50468

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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