We narrowed to 14,262 results for: RON

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pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 I136A, I139A, M142A

Plasmid

#74617

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-C. thermophilum LIC

Plasmid

#74780

Purpose

encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

dynein light intermediate chain (Chaetomium thermophilum)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pExp_TRE:TAL21

Plasmid

#51795

Purpose

Inducible expression of TAL21 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL21

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

Plasmid

#60393

Purpose

Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast, Synthetic)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB2a Cterminal tail

Plasmid

#60396

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP2 CC

Plasmid

#59040

Purpose

for baculovirus expression of CAMSAP2 CC in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC domain (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP3 CC

Plasmid

#59042

Purpose

for baculovirus expression of mouse CAMSAP3 CC in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CC domain (Camsap3 Mouse)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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