We narrowed to 11,231 results for: SHA

1. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

   Plasmid

   #166809

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS04 [myo-3p::bPGC::SL2::mCherry]

   Plasmid

   #168172

   Purpose

   Expression of bPGC in BWMs of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   bPGC
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DMGDH

   Plasmid

   #155549

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DMGDH (DMGDH Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DDX56

   Plasmid

   #155530

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DDX56 (DDX56 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CIR1

   Plasmid

   #155452

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CIR1 (CIR1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BTG4

   Plasmid

   #155428

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   BTG4 (BTG4 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RBP2-like-bio

   Plasmid

   #68536

   Purpose

   Expresses full-length P. vivax RBP2-like (PVX_101590) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

   Depositor

   Julian Rayner, Gavin Wright

   Article

   Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

   Insert

   RBP2-like
   

   Use

   Tags

   rat CD4 d3+4, enzymatic biotinylation sequence

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-7 asyn S87A

   Plasmid

   #36058

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

   Insert

   alpha-synuclein S87A (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S87A

   Promoter

   T7

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

   Plasmid

   #166801

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   S730A

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-BASIC- ARHGAP29 Mutant PROMOTER

    Plasmid

    #104156

    Purpose

    luciferase reporter with ARHGAP29 mutant promoter

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 promoter (ARHGAP29 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    delete two TEAD binding sites

    Promoter

    ARHGAP29

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DLG3_PDZ_3

    Plasmid

    #103948

    Purpose

    Protein expression and purification of NE-DLG PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28A-p53 (1-73) (P to A)

    Plasmid

    #62069

    Purpose

    Expression of human p53 (1-73) (all P to A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; all Prolines changed …

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. PTPN13_PDZ_3

    Plasmid

    #103900

    Purpose

    Protein expression and purification of PTPN13 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H14-MBP-SUMO-Cys-Linker-BAF_L58R

    Plasmid

    #101780

    Purpose

    Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Tags

    His14-MBP-SUMO

    Expression

    Bacterial

    Mutation

    L58R mutation

    Promoter

    T5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW TetO NET1A-V5 WT

    Plasmid

    #69828

    Purpose

    Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAGI1_PDZ_2

    Plasmid

    #103899

    Purpose

    Protein expression and purification of MAGI1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pECE M2-SH3PXD2A 8 serines mutated to alanines

    Plasmid

    #69816

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

    Plasmid

    #166796

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    KK93EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

    Plasmid

    #166800

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    E597R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSN67

    Plasmid

    #100124

    Purpose

    expresses putative KLF4 DBD with a 6xHis tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of KLF4

    Promoter

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pfast bac Rbx-HA

    Plasmid

    #51893

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Rbx1 (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pGL3-158/+19

    Plasmid

    #35541

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL3-110/+19

    Plasmid

    #35542

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pECE M2-SH3PXD2A 6 serines mutated to alanines

    Plasmid

    #69815

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. integrin beta6 777T pBabe puro

    Plasmid

    #13583

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA 3.2/V5-DEST NET1A S46A

    Plasmid

    #69818

    Purpose

    Expresses NET1A-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S46A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HLH Bmal Pas 1-465-VenC I317D

    Plasmid

    #47367

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, I317D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only