Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5181 - 5200 of 65358 results

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pRZ336:CMVp-EC-Ex1-(5'D)-miRS1-(3'SE)-BD6-S-pA

Plasmid

#251328

Purpose

Expression of ECFP-Ex1 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

EC-Ex1

Expression

Mammalian

Promoter

CMVp

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ337:CMVp-mK2-Ex1-(5'D)-miRS2-(3'SE)-BD5-S-pA

Plasmid

#251329

Purpose

Expression of mK2-Ex1 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

CMVp

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Publication

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV-HA-ENO1-NU

Plasmid

#255450

Purpose

Mammalian expression of HA-tagged human ENO1

Depositor

Christine Mayr

Publication

Luo et al Cell. 2026 Jun 8:S0092-8674(26)00576-3.

Insert

ENO1 (ENO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-ENO1-U

Plasmid

#255451

Purpose

HA-tagged human ENO1 with its own 3'UTR

Depositor

Christine Mayr

Publication

Luo et al Cell. 2026 Jun 8:S0092-8674(26)00576-3.

Insert

ENO1 (ENO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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PHB1_BirA*

Plasmid

#251206

Purpose

Expresses human PHB1 tagged with BirA* in mammalian cells

Depositor

Svetlana Konovalova

Insert

Prohibitin 1 (PHB1 Human)

Use

Retroviral

Tags

BirA*

Expression

Mammalian

Available since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-beta/alpha-14-3-3

Plasmid

#250062

Purpose

Bacterial expression of beta/alpha 14-3-3

Depositor

Mark Bedford

Publication

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Beta/Alpha (YWHAB Human)

Expression

Bacterial

Mutation

None

Available since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-8a)

Plasmid

#241069

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,L452R,F490R,Q493S,S494R,Q498Y,K529L,T…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-9a)

Plasmid

#241070

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-9a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-9a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R346T,L452R,F490R,Q493S,S494R,Q498Y,K…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-5a)

Plasmid

#241067

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147N,S155R,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-7a)

Plasmid

#241068

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-7a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-7a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147N,S155R,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4a)

Plasmid

#241065

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,P25del,P26del,A27S,T76I,G142D,M153S,V213G,G3…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4b)

Plasmid

#241066

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,T76I,G142D,M153S,V…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2g)

Plasmid

#241064

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2g) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2g) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S155R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2e)

Plasmid

#241062

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2e) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2e) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K529L,T19I,L24del,P25del,P26del,A27S,G142D,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2f)

Plasmid

#241063

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2f) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2f) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K147N,T19I,L24del,P25del,P26del,A27S,G142D,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2c)

Plasmid

#241060

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2d)

Plasmid

#241061

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

F490R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2a)

Plasmid

#241058

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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