We narrowed to 59,254 results for: %s

1. pTET2-lucCP-3MS2

   Plasmid

   #198351

   Purpose

   Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Firefly Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   pTET2

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDL691

   Plasmid

   #231166

   Purpose

   T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

   Depositor

   Daniel Voytas

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

   Insert

   ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
   

   Expression

   Plant

   Promoter

   PEBV sub-genomic

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_FKBP-V5-TWIST1

   Plasmid

   #215022

   Purpose

   AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human TWIST1

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Use

   AAV

   Tags

   FKBP-V5

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-UAP56

   Plasmid

   #157661

   Purpose

   Expresses UAP56 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Spliceosome RNA helicase DDX39B (DDX39B Human)
   

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-43

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/myc/ER-αT9ib

   Plasmid

   #110768

   Purpose

   Expresses ER intrabody against mouse and human TLR9

   Depositor

   Thomas Böldicke

   Article

   Reimer et al Cell Mol Biol Lett. 2013 Sep;18(3):433-4

   Insert

   intrabody against mouse and human TLR9
   

   Tags

   ER retention signal (SEKDEL), ER signal peptide, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn1 CaRhGC wt T2A tDimer

   Plasmid

   #101720

   Purpose

   humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhGC
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn1 and ribosomal skip sequence T2A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NRCH-APOBEC-nCas9-Ung

   Plasmid

   #198552

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6_sgRNA_CAG_hSt1Cas9_LMD9

   Plasmid

   #110626

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH3626

    Plasmid

    #179630

    Purpose

    Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Tags

    RFP

    Expression

    Worm

    Promoter

    Pacr-2(s)

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHW581

    Plasmid

    #198818

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5 TEX264wt-cSSH

    Plasmid

    #220209

    Purpose

    Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SYMPK

    Plasmid

    #156071

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SYMPK (SYMPK Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MIGR1_FLAG_TAL1-long_GFP

    Plasmid

    #210638

    Purpose

    Overexpression of TAL1-long

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1-short (TAL1 Human)
    

    Use

    Retroviral

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCKO_LacZ_sgRNA

    Plasmid

    #74179

    Purpose

    lentiviral vector expressing sgRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    LacZ sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTET2-RlucCP

    Plasmid

    #198352

    Purpose

    Part of Firefly reporter system, encodes Renilla luciferase

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130993

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3 Pro Mut E2

    Plasmid

    #48750

    Purpose

    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    

    Use

    Luciferase

    Mutation

    Mutated E-box2 sequence from CACGTT to GCTAGT

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. PRPF18

    Plasmid

    #155846

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PRPF18 (PRPF18 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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