We narrowed to 12,758 results for: sequence

1. Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R]

   Plasmid

   #177539

   Purpose

   Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human). Derived from hybridoma N37A/10.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-Kvbeta1.2/KCNAB1 K+ channel [K47/42R]

   Plasmid

   #177441

   Purpose

   Mammalian Expression Plasmid of anti-Kvbeta1.2/KCNAB1 K+ channel (Human). Derived from hybridoma K47/42.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Kvbeta1.2/KCNAB1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNAB1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-MAGT1_STOP

   Plasmid

   #161468

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MAGT1 (MAGT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCJ200

   Plasmid

   #162673

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C and a sequence Y75-F104 to introduce a 6th…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ210

   Plasmid

   #162683

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C, G489C, S530C, and a sequence Y75-F104 to i…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.2_mCherry_mito Grx1-roGFP2

   Plasmid

   #155044

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   mitochondrial targeting sequence and roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS-memb-mCherry

   Plasmid

   #53750

   Purpose

   Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   2x membrane linker
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3 HA C9ORF72 op

    Plasmid

    #74156

    Purpose

    Express HA tagged optimized C9ORF72 in mammalian cells

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    C9ORF72 (C9orf72 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    optimized sequence

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTALYM3B15

    Plasmid

    #47878

    Purpose

    TALE-mClover against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. mKeima-Red-Golgi-7

    Plasmid

    #56014

    Purpose

    Localization: GalT Targeting Sequence, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mKeima-Red

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. AICSDP-80:UBTF-HaloTag

    Plasmid

    #133963

    Purpose

    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF

    Depositor

    Allen Institute for Cell Science

    Insert

    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    

    Use

    CRISPR

    Tags

    HaloTag-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTALYM3-Telo1

    Plasmid

    #47883

    Purpose

    TALE-mClover against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSPneoHHgRNAH

    Plasmid

    #63557

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU1_000

    Plasmid

    #186773

    Purpose

    * Engineered U1 snRNA plasmid for insertion of guide sequences.

    Depositor

    Eugene Yeo

    Article

    Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U1 Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTALYM16-Telo1

    Plasmid

    #47882

    Purpose

    TALE-mRuby2 against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. poRibo-T2

    Plasmid

    #69347

    Purpose

    Ribo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutation

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    oRibo-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69546

    Purpose

    Tet-On system expressing MyoD-2A-GFP-2xNLS.

    Depositor

    Charles Gersbach

    Inserts

    eGFP
    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Removed stop codon to fuse it to the 2A-eGFPnls i…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAd/CMV/DAX1-IRES-nEGFP

    Plasmid

    #29752

    Depositor

    Edward McCabe

    Insert

    dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (NR0B1 Human)
    

    Use

    Tags

    EGFP, IRES, and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    No mutations; Reporter is nuclear localized EGFP;…

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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