Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 10,457 results for: T7

Showing: 10401 - 10440 of 10457 results

  18. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

    Enlarge

  21. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

    Enlarge

  22. pTXBI-mclover-YTHDC1-FL

    Plasmid
    #177121
    Purpose
    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177121-plasmid-map-sequence-id-348688

    Enlarge

  30. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    229539-plasmid-map-sequence-id-456666

    Enlarge

  31. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Use
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

    Enlarge

  33. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

    Enlarge

  37. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

    Enlarge

  39. pTXBI-mclover-YTH-IDR2

    Plasmid
    #177122
    Purpose
    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH and IDR2 domains only (IDR1 deletion)
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177122-plasmid-map-sequence-id-348676

    Enlarge

Showing: 10401 - 10440 of 10457 results