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pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

pCMV and T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPuroR-GMRWhite

Plasmid

#165884

Purpose

Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdc20

Plasmid

#160954

Purpose

Cdc20 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdc20 shRNA (Cdc20 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shSpc25

Plasmid

#160960

Purpose

Spc25 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Spc25 shRNA (Spc25 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCenpf

Plasmid

#160956

Purpose

Cenpf shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cenpf shRNA (Cenpf Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shNuf2

Plasmid

#160961

Purpose

Nuf2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nuf2 shRNA (Nuf2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI126

Plasmid

#126066

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI127

Plasmid

#126067

Purpose

Module 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI177

Plasmid

#126077

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP1-10

Use

Binary, module 1

Tags

linker (GTGGGGS)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI129

Plasmid

#126210

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI161

Plasmid

#126211

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FDHFR_WTTS

Plasmid

#91237

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42F

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FG121VDHFR_R166QTS

Plasmid

#91238

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42FG121 and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_M42FG121VDHFR_WTTS

Plasmid

#91239

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

M42FG121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_R166QTS

Plasmid

#91240

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

R166Q and W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTEcoliDHFR_R166QTS

Plasmid

#91242

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

R166QTS

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_R166QTS

Plasmid

#91243

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N and R166Q

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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