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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,371 results for: BLI

Showing: 5201 - 5220 of 8371 results

  1. MACROD1

    Plasmid
    #39041
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MACROD1 (MACROD1 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. pGEX6P1-DEST-HA

    Plasmid
    #119751
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119751-plasmid-map-sequence-id-234027

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  5. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid
    #140030
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140030-plasmid-map-sequence-id-275866

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  6. GFP-I48 ELP

    Plasmid
    #67857
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residues
    Depositor
    Andrew MacKay
    Insert
    GFP fused to ELP (VPGIG) repeated 48 times
    Use
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67857-plasmid-map-sequence-id-117815

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  7. p2Bac.V5.p62

    Plasmid
    #39218
    Depositor
    Aziz Sancar
    Insert
    p62 (GTF2H1 Human)
    Use
    Tags
    V5
    Expression
    Insect
    Mutation
    Promoter
    Pp10
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107305-plasmid-map-sequence-id-204646

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  9. APPL1-N308D-GFP

    Plasmid
    #102783
    Purpose
    Point mutation in APPL1 that diminishes its interaction with Rab5
    Depositor
    Donna Webb
    Insert
    APPL1 (APPL1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed asparagine 308 to aspartate; *(see below)
    Promoter
    CMV
    Available since
    Jan. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    102783-plasmid-map-sequence-id-198005

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  10. ACSM2B

    Plasmid
    #39167
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACSM2B (ACSM2B Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Actually the mitochondrial form, GI:58082049, wit…
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pET28-Strep-TEV-Nsp14

    Plasmid
    #154955
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    Strep-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154955-plasmid-map-sequence-id-307850

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  12. Aiolos AA130-189 in Pea

    Plasmid
    #74459
    Purpose
    Monitor post-translational degradation of Aiolos AA130-189 via EGFP:mCherry ratio (Lentiviral SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry)
    Depositor
    Benjamin Ebert
    Insert
    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    74459-plasmid-map-sequence-id-140419

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  13. HOXD9 (human) pET

    Plasmid
    #8570
    Depositor
    Corey Largman
    Insert
    HOXD9 (HOXD9 Human)
    Use
    Tags
    T7
    Expression
    Bacterial
    Mutation
    PCR human cDNA clone; makes protein by in vitro T…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    8570-plasmid-map-sequence-id-208949

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  14. pLEX307-CTSS-G418

    Plasmid
    #158471
    Purpose
    Constitutive lentiviral expression of human CTSS.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSS (CTSS Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158471-plasmid-map-sequence-id-308129

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  15. pDONR221-CTSS

    Plasmid
    #158445
    Purpose
    Gateway Cloning compatible entry vector for the human CTSS gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSS (CTSS Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158445-plasmid-map-sequence-id-308884

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  16. ACADVL

    Plasmid
    #38838
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACADVL (ACADVL Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. ARHGAP15

    Plasmid
    #38903
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ARHGAP15 (ARHGAP15 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. CENTG1

    Plasmid
    #39144
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CENTG1 (AGAP2 CENTG1, Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. FASN

    Plasmid
    #38793
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FASN (FASN Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. GSK-3beta S9A (Human)/pCS2+

    Plasmid
    #16334
    Depositor
    Peter Klein
    Insert
    glycogen synthase kinase-3 beta (GSK3B Human)
    Use
    Vertebrate expression
    Tags
    Myc
    Expression
    Mutation
    GSK-3 beta with serine-9 to alanine point mutation
    Promoter
    Available since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 5201 - 5220 of 8371 results