We narrowed to 2,784 results for: aen

1. pTD81

   Plasmid

   #172902

   Purpose

   Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

   Depositor

   David Reiner

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   TIR-1 (TIR1 Mustard Weed)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMT3-FBF2 SS/Y

   Plasmid

   #166973

   Purpose

   Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMT3-FBF2 AS/Y

   Plasmid

   #166974

   Purpose

   Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST40-2XHA-Src-S4-UD-mCherr

   Plasmid

   #140307

   Purpose

   Expresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   contains only the SH4 and UD regions fused to mCh…

   Promoter

   CMV

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-2XFL-Src-S32

   Plasmid

   #140309

   Purpose

   Expresses Flag-tagged human Src-S32 in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   contains only the SH3 and SH2 domains

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR13

   Plasmid

   #69155

   Purpose

   read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   swsn-1 (swsn-1 Nematode)
   eGFP
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, codon-optimzed index 1.…

   Promoter

   rps-27 and swsn-1

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. RGS-6xHis-LIN-29-pcDNA3.1-

   Plasmid

   #52515

   Purpose

   expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Lin-29 b (lin-29 Nematode)
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   wt (changed coding sequence in 5' (5th codon…

   Promoter

   CMV

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Use

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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