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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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CLTA-mCherry HDRT Source (pTR 177)

Plasmid

#112017

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-mCherry HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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BATF-GFP HDRT Source (pTR 146)

Plasmid

#112015

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

BATF-GFP HDRT (BATF Human)

Use

CRISPR and Synthetic Biology

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RAB11A-BFP HDRT Source (pTR 145)

Plasmid

#112014

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

RAB11A-BFP HDRT (RAB11A Human)

Use

CRISPR and Synthetic Biology

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-N MCV ER

Plasmid

#37860

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

MCV ER (MCPyV_gp2, MCPyV_gp1 Human Merkel Cell Virus)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
CD4-C-BFP HDRT Source (pTR 176)

Plasmid

#112020

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-BFP HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CD4-C-mCherry HDRT Source (pTR 175)

Plasmid

#112019

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CD4-mCherry HDRT (CD4 Human)

Use

CRISPR and Synthetic Biology

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR53:1-358

Plasmid

#211256

Purpose

Mammalian expression of full-length human WDR53. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR53:M1-F358 (WDR53 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR70:1-654

Plasmid

#211143

Purpose

Mammalian expression of full-length human WDR70. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR70:M1-I654 (WDR70 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RBBP7:1-425

Plasmid

#211113

Purpose

Mammalian expression of full-length human RBBP7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP7:M1-S425 (RBBP7 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDR38:1-314

Plasmid

#211329

Purpose

Mammalian expression of full-length human WDR38. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR38:M1-T314 (WDR38 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1153

Plasmid

#29068

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple36 (CRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1156

Plasmid

#29071

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple39 (CRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1154

Plasmid

#29069

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple37 (CRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_WDR20:1-569

Plasmid

#211136

Purpose

Mammalian expression of full-length human WDR20. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR20:M1-P569 (WDR20 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_DNAAF10:1-357

Plasmid

#211138

Purpose

Mammalian expression of full-length human DNAAF10. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DNAAF10:M1-I357 (WDR92 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_PPWD1:1-646

Plasmid

#211116

Purpose

Mammalian expression of full-length human PPWD1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PPWD1:M1-K646 (PPWD1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDR70:1-654

Plasmid

#211184

Purpose

Mammalian expression of full-length human WDR70. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR70:M1-I654 (WDR70 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_WDR20:1-569

Plasmid

#211177

Purpose

Mammalian expression of full-length human WDR20. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR20:M1-P569 (WDR20 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_DNAAF10:1-357

Plasmid

#211179

Purpose

Mammalian expression of full-length human DNAAF10. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DNAAF10:M1-I357 (WDR92 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge