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FB272

Plasmid

#203611

Purpose

Coding sequence of pyr4 gene from T. reesei.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

pyr4

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-W279A-short-UBASH3A-Ctag

Plasmid

#195402

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Mutation

W279A

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ctag

Plasmid

#195399

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Gateway donor vector

Mutation

K202R

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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K202R-short-UBASH3A-V5-6xHis

Plasmid

#195406

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

K202R

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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W279A-short-UBASH3A-V5-6xHis

Plasmid

#195409

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5-6xHis

Expression

Mammalian

Mutation

W279A

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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V5-K202R-short-UBASH3A

Plasmid

#192084

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-W279A-short-UBASH3A-Ntag

Plasmid

#192096

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ntag

Plasmid

#192094

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Mutation

K202R

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ctag

Plasmid

#192099

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ntag

Plasmid

#192100

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-sfGFP

Plasmid

#180756

Purpose

pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRNA2

Plasmid

#174005

Purpose

Used to generate stable mRNA for heterologous expression experiments (no polyA)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM862

Plasmid

#169085

Purpose

MoClo level 0 BvDODA1 coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvDODA1

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM1021

Plasmid

#169084

Purpose

MoClo level 0 BvCYP76AD1 coding sequence module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvCYP76AD1

Use

Synthetic Biology; Moclo cloning vector

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 221-PHL2 (STOP)

Plasmid

#118683

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Other

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 221-XERICO (STOP)

Plasmid

#118685

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Other

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST 527-PHL2

Plasmid

#118670

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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