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pX459-dNrg1

Plasmid

#173696

Purpose

A knockout vector for the dog Nrg1.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEreg

Plasmid

#173697

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dTgfa

Plasmid

#173698

Purpose

A knockout vector for the dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dHbegf

Plasmid

#173699

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-dEgf

Plasmid

#173700

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-dEgf

Plasmid

#173840

Purpose

A knock-out vector for the dog Egf

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-puro-dHbegf

Plasmid

#173841

Purpose

A knockout vector for the dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-dEreg

Plasmid

#173843

Purpose

A knockout vector for the dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-puro-dEgfr

Plasmid

#173844

Purpose

A knockout vector for the dog Egfr.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Egfr gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisHbegf

Plasmid

#173846

Purpose

Encoding dog Hbegf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Hbegf.

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisTgfa

Plasmid

#173847

Purpose

Encoding dog Tgfa.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEreg

Plasmid

#173848

Purpose

Encoding dog Ereg.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Tgfa.

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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C513-E06: attB5r-pA-polyhedrinp>-attB1r

Plasmid

#162948

Purpose

Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter with upstream polyA sequence

Use

Synthetic Biology

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_S176E

Plasmid

#131203

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 S176E (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S176E

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-RIP2_Y474F

Plasmid

#131204

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 Y474F (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Y474F

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-TTM

Plasmid

#127794

Purpose

Expresses the TTM/CYTH domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 1-200)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLV(shRNA)-Puro-6 CCEPR Vb2

Plasmid

#108278

Purpose

shRNA for CCEPR lncRNA

Depositor

Karl Munger

Article

Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

Insert

CCEPR (CCEPR Human)

Use

Lentiviral

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-ScSEIPIN-Ndelta

Plasmid

#96985

Purpose

Express yeast SEIPIN gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

ScSEIPIN (SEI1 Budding Yeast)

Tags

6xHis and GFP

Expression

Plant

Mutation

N-terminal 39 nucleotides were deleted from yeast…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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