We narrowed to 143,240 results for: ins

1. pET28 mid.7

   Plasmid

   #191900

   Purpose

   Encodes a GFP design, mid.7

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   mid.7
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   S72T Y145F V224I

   Promoter

   T7

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2XMyc-LRRK2-WD40

   Plasmid

   #25073

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Contains only the WD40 domain aa 2155-2527

   Promoter

   Available since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMTH PKC zeta

   Plasmid

   #8414

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Mouse Protein Kinase C zeta (Prkcz Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pJRoC30_VP5H

   Plasmid

   #188061

   Purpose

   PROSS-designed versatile peroxidase 5H

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

   Insert

   PROSS-designed versatile peroxidase from Pleurotus ostreatus
   

   Use

   Tags

   S. cerevisiae native α-factor prepro-leader signa…

   Expression

   Yeast

   Mutation

   43 PROSS mutations, described in publication

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX SHIP2-SH2

   Plasmid

   #46493

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHIP2
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 8-131

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-ZZ-TEV-SNAPf-BICD2N(1-400)

   Plasmid

   #111857

   Purpose

   N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICD2N(1-400)
   

   Use

   Tags

   His-tag, SNAPf-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   Optimised for Sf9 expression

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-PI(WT)-Src(KD)-mCherry

   Plasmid

   #87358

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src (Src Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   D388R

   Promoter

   CMV

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNP1 tdTomato toehold switch

   Plasmid

   #107354

   Purpose

   Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 5 + tdTomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 2XMyc-LRRK2-COR

    Plasmid

    #25069

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the COR domain aa1408-1850

    Promoter

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET28a Gx-NL

    Plasmid

    #169105

    Purpose

    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGen2.1-redCALWY-4

    Plasmid

    #50738

    Purpose

    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-4 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-ReaChR-citrine

    Plasmid

    #169045

    Purpose

    Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-citrine
    

    Use

    Gateway entry cloning vector

    Tags

    citrine

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX PLCg1(C)-SH2

    Plasmid

    #46469

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg1(C)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 656-766

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2279

    Plasmid

    #70706

    Purpose

    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    D10A and H557A in SaCas9

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE puro mouse IRS-2

    Plasmid

    #11371

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE bleo human IRS-1

    Plasmid

    #11448

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pGen2.1-redCALWY-1

    Plasmid

    #50739

    Purpose

    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

    Insert

    gene for redCALWY-1 Zn2+ sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Syk(NC)-SH2

    Plasmid

    #46521

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(NC) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-263

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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