We narrowed to 150,160 results for: ins

Showing: 5221 - 5240 of 150160 results

Results per page:

Sort results by:

pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRE4-Azi

Plasmid

#105829

Purpose

Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

Depositor

Irene Coin

Article

Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

Inserts

Humanized EAziRS
4 copies of the U6-BstYam tRNA cassette

Expression

Mammalian

Mutation

(Y37L, D182S, F183M, L186A, D265R)TyrRS

Promoter

PGK and U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-ReaChR-citrine

Plasmid

#169045

Purpose

Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-citrine

Use

Gateway entry cloning vector

Tags

citrine

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-hLC3B-pcDNA3.1

Plasmid

#40827

Depositor

David Rubinsztein

Article

Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

Insert

LC3B (MAP1LC3B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

Plasmid

#167942

Purpose

Inducible NKX2-1 overexpression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

TTF1 (NKX2-1 Human)

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
VPS13A^Halo

Plasmid

#118759

Purpose

VPS13A internally tagged with Halo at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

Halo tag inserted at position 1372

Expression

Mammalian

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-80L MBP-SspB Micro

Plasmid

#60410

Purpose

E. coli expression of SspB Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB micro

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

R73Q, Y11K and A15E

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-80L iLID (C530M)

Plasmid

#60408

Purpose

E. coli expression of iLID (C530M)

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

iLID C530M

Tags

His

Expression

Bacterial

Mutation

C530M

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
VSVG-mCherry-RUSH

Plasmid

#203647

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

VSVG

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE puro human IRS-1

Plasmid

#11025

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-HcKCR2-EYFP

Plasmid

#182022

Purpose

Express light-gated potassium channel HcKCR2-EYFP in mammalian cells

Depositor

Mingshan Xue

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-X1-Neo-GFP-FAM134B-LIRmut

Plasmid

#109027

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

FAM134B-LIRmut (RETREG1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

Promoter

EIF-1a promoter

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge