We narrowed to 11,787 results for: nsf

1. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219686

   Purpose

   Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1167A

   Plasmid

   #218233

   Purpose

   HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene

   Depositor

   Omar Akbari

   Insert

   putative metabolite transport protein
   

   Expression

   Insect

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

   Plasmid

   #205417

   Purpose

   Mutagenesis of Ntsr1, second guide

   Depositor

   Larry Zweifel

   Insert

   Ntsr1 (Ntsr1 Mouse)
   

   Use

   AAV and CRISPR

   Promoter

   U6

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-MP-84-spCas9-U6-gRNA-tlock

   Plasmid

   #206936

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   MP-84

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hPhyB621(Y276H)-P2A-m1V

   Plasmid

   #197377

   Purpose

   The AAV vector for PhyB621(Y276H) and m1V

   Depositor

   Michiyuki Matsuda

   Insert

   Phytochrome B Y276H(1-621 aa)
   

   Use

   AAV

   Tags

   P2A-m1V

   Mutation

   Human codon optimized PhyB (1-621 aa), Y276H muta…

   Promoter

   CMV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

   Plasmid

   #194249

   Purpose

   Expresses 3 non-targeting miRNAs

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRNegative
   

   Use

   AAV and RNAi

   Promoter

   CAG

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1X-UASTattB

   Plasmid

   #189861

   Purpose

   Fly cloning and transformation

   Depositor

   Jung Hwan Kim

   Article

   Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENN.AAV.SV40-si-MAPRE1

   Plasmid

   #187862

   Purpose

   a human siRNA-expressing plasmid targeting MAPRE1

   Depositor

   Yan Zeng

   Insert

   si-MAPRE1 (MAPRE1 Human)
   

   Use

   AAV

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2iD6Hoxa13bER4(#686)

   Plasmid

   #184101

   Purpose

   cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   ZfHoxa13b-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2iD6Hoxa13aER4(#685)

    Plasmid

    #184100

    Purpose

    cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfHoxa13a-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. J-GALTA-3

    Plasmid

    #166523

    Purpose

    scFv of a human scaffold targeting Galactose-1-phosphate uridylyltransferase. Antigen coverage aa 1-379 of 379, full-length

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. A-PRMT3-3

    Plasmid

    #166514

    Purpose

    scFv of a human scaffold targeting Protein arginine methyltransferase 3 isoform 1. Antigen coverage aa 211-531 of 531.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid

    #159907

    Purpose

    Mutagenesis of Netrin1

    Depositor

    Larry Zweifel

    Insert

    Netrin1 (Ntn1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTU1-C-cat

    Plasmid

    #112791

    Purpose

    Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    cat
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid

    #178429

    Purpose

    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    Edward Callaway

    Inserts

    TVA950 Glu66→Thr
    Optimized G
    EGFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Promoter

    hSyn

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOttc556 - pAAV EF1a iCre

    Plasmid

    #210416

    Purpose

    AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

    Depositor

    Christopher Richie, Brandon Harvey

    Insert

    iCre
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-tdTomato (codon diversified)

    Plasmid

    #59462

    Purpose

    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

    Depositor

    Edward Boyden

    Has Service

    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    tdTomato
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    codon diversified

    Promoter

    CAG

    Available Since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid

    #177672

    Purpose

    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVLINK-EF1alpha-5' ROBO2

    Plasmid

    #236686

    Purpose

    5' AAVLINK plasmid for ROBO2 expression

    Depositor

    Zhonghua Lu

    Insert

    ROBO2 (ROBO2 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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