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L4427 IL-10Ra CTEVp chain with cJun in TUPV3

Plasmid

#244194

Purpose

Expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-hcJun

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and human cJun (JUN Synthetic, Human)

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

CTEVp_190K

Promoter

hEF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pPBneo-CIBN-lin-p53CT(98-393)-R273H-mNeonGreen-NLSx3

Plasmid

#241849

Purpose

Expresses a negative control of the localizer of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CIBN and mNeonGreen

Expression

Mammalian

Mutation

p53 C-terminus (97-393 aa) containing R273H mutat…

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-L22Q/W23S/W53Q/F54S-lin-hCRY2

Plasmid

#241847

Purpose

Expresses a negative control of the actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

N-terminus (1-97 aa) containing L22Q/W23S/W53Q/F5…

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLOC-D133p53a

Plasmid

#241916

Purpose

Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.

Depositor

Curtis Harris

Article

Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. d

Insert

Delta133p53alpha (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-C73 (pGEX4T1)

Plasmid

#238428

Purpose

Expresses GRP78 with the only the last 73 amino acids at the C terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C17 (pGEX4T1)

Plasmid

#238426

Purpose

Expresses GRP78 with the last 17 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C11(pGEX4T1)

Plasmid

#238425

Purpose

Expresses GRP78 with the last 11 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta C73 (pGEX4T1)

Plasmid

#238427

Purpose

Expresses GRP78 with the last 73 amino acids at the C terminus deleted fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta KDEL (pGEX4T1)

Plasmid

#238424

Purpose

Expresses GRP78 Delta KDEL fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_RPP20 (pAVA3546)

Plasmid

#239316

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RPP20

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting RPP20 (POP7 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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p-HITI-F9-Ckm

Plasmid

#226120

Purpose

HITI insert construct with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKLV2-U6gRNA5(hEGFR_2b)-PGKpuro2ABFP-W

Plasmid

#208432

Purpose

Lentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

EGFR (EGFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hEGFR_1k)-PGKpuro2ABFP-W

Plasmid

#208431

Purpose

Lentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

EGFR (EGFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

Plasmid

#236278

Purpose

Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.

Depositor

Arnab Mukherjee

Article

Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

Insert

human aquaporin 1 (AQP1 Human)

Use

Lentiviral

Tags

Flag and fkbp12

Expression

Mammalian

Promoter

CMVtight

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPGrp

Plasmid

#236002

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPGrp (Cyth3 Synthetic, Rat)

Tags

Cerulean, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(WT)

Plasmid

#215450

Purpose

Gateway entry vector with integrin beta1 wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway Cloning

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Gfa104-hIRβ

Plasmid

#236227

Purpose

Expressing a truncated, constitutively active human insulin receptor (IRβ) in astrocyte

Depositor

Olivier Thibault

Article

Frazier et al Neurochem Res. 2019 Jan;44(1):269-280. d

Insert

human Insulin Receptor beta subunit (INSR Human)

Use

AAV

Expression

Bacterial

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-TAZ-CAMTA1

Plasmid

#235679

Purpose

Express TAZ-CAMTA1 fusion gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

Insert

TAZ-CAMTA1 (WWTR1, CAMTA1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Promoter

None

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAND1-AM-Tag-PGK-Neo

Plasmid

#223216

Purpose

Targeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Cre/Lox; Human targeting

Tags

AM-Tag

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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