We narrowed to 9,648 results for: Ada;

1. pCRISPR-aBEST

   Plasmid

   #131464

   Purpose

   Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

   Inserts

   Streptomyces codon optimized spCas9n (D10A)
   streptomyces codon optimized tadA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   tipA

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcsII-MECL1-flag

   Plasmid

   #86774

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   immunoproteasome beta subunit 2 (MECL1) (PSMB10 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   unknown

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGLO-GFP-1UAG

   Plasmid

   #82500

   Purpose

   Expresses GFP with 1 UAG codon at the amino acid position 3

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescent Protein with 1 UAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3

   Promoter

   AraBAD

   Available since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-6XHis-SUMO-SIRT2

   Plasmid

   #102622

   Purpose

   Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Tags

   6XHis-SUMO

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-37 and 357-389

   Promoter

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1449 SED1-Lenti

   Plasmid

   #180824

   Purpose

   SED1 biosensor for stable integration and expression in mammalian cells

   Depositor

   Rajat Rohatgi

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 osmosensor
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-IP-EGFP-hAtg13

   Plasmid

   #38191

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLKO.1-TRC.mKO2

   Plasmid

   #85208

   Purpose

   Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1_MeCP2(WT)

   Plasmid

   #110186

   Purpose

   Expression of mouse MeCP2 (WT) in mammalian cells

   Depositor

   Adrian Bird

   Article

   Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

   Insert

   MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-PITCh-VIM

   Plasmid

   #227301

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of VIM (VIM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGPTVII-nlsSED1

    Plasmid

    #178190

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. iCAP-BI-28-KanR

    Plasmid

    #203532

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI28 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDicAID_nCas9-PmCDA_NptII_Della

    Plasmid

    #91694

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid

    #154141

    Purpose

    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Use

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    R887E

    Promoter

    SFFV

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNKX2-5-T2A-GFP-PGK-Puro

    Plasmid

    #203607

    Purpose

    Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

    Depositor

    Matthew Birket

    Article

    Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

    Insert

    NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Target-ACEmax

    Plasmid

    #139105

    Purpose

    Mammalian Target-ACEmax expression vector (pSI-625)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBMN YFP-LID

    Plasmid

    #31767

    Depositor

    Thomas Wandless

    Article

    Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

    Insert

    YFP-LID (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

    Promoter

    LTR

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-HisMBP-SED1

    Plasmid

    #178191

    Purpose

    SED1 biosensor for expression in Escherichia coli

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSH-EFIRES-B-AtAFB2-mCherry

    Plasmid

    #129718

    Purpose

    AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_OTmut

    Plasmid

    #185388

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP Abi1

    Plasmid

    #74905

    Purpose

    Expresses human Abi1 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

    Insert

    Abi1 (ABL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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